Description

Kanamycin 6'-Acetyltransferase From Escherichia Coli is a highly purified enzyme that catalyzes the transfer of an acetyl group to the 6' position of the aminoglycoside antibiotic kanamycin. This specific enzymatic activity is critical for research into antibiotic resistance mechanisms and for the development of analytical assays. It is an essential reagent for molecular biology laboratories, pharmaceutical R&D departments, and academic institutions focused on microbiology and enzymology.

Application

• Antibiotic Resistance Research: Used as a model enzyme to study the molecular basis of aminoglycoside modification and bacterial resistance.
• Biochemical Assay Development: Serves as a key component in developing high-throughput screening assays for potential acetyltransferase inhibitors.
• Enzymology Studies: Employed in kinetic studies to understand substrate specificity and catalytic mechanism of bacterial acetyltransferases.
• Molecular Biology Tool: Utilized in controlled experiments for the in vitro acetylation of kanamycin and related compounds.
• Pharmaceutical Quality Control: Can be used in reference methods to detect and quantify enzymatic modification of aminoglycoside antibiotics.
• Educational & Training Purposes: Provides a well-characterized enzyme for teaching advanced concepts in biochemistry and microbiology.

Basic Information

Product Name	Kanamycin 6'-Acetyltransferase From Escherichia Coli
CAS No.	62213-49-4
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	AAC(6')-I; Kanamycin 6'-N-acetyltransferase; Aminoglycoside 6'-N-acetyltransferase type I; 6'-AAC; AAC6'; Kanamycin acetyltransferase; Antibiotic-modifying enzyme; EC 2.3.1.82
EINECS	Contact for details

Quality Control

Our Kanamycin 6'-Acetyltransferase is produced and tested under stringent conditions to ensure high purity, specific activity, and batch-to-batch consistency. Each lot is characterized for protein content, enzymatic activity, and absence of contaminating nucleases or proteases. A comprehensive Certificate of Analysis (COA) detailing purity (by SDS-PAGE), specific activity (units/mg protein), buffer composition, and storage conditions is provided with every shipment to support your research and compliance needs.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store frozen at -20°C or below. Avoid repeated freeze-thaw cycles. The product is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use to prevent moisture absorption.

Specification

Item	Specification
Appearance	Clear to slightly opalescent solution
Identification (Activity)	Catalyzes acetylation of kanamycin
Protein Concentration	≥ 1 mg/mL (Bradford assay)
Specific Activity	≥ 50 units/mg protein
Purity (SDS-PAGE)	≥ 90%
Buffer	Tris-HCl, Glycerol, DTT
pH	7.5 ± 0.5
Endotoxin Level	< 1.0 EU/μg (where specified)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.