Description

Thrombin is a highly specific serine protease enzyme that plays a critical role in the final stage of the blood coagulation cascade by converting soluble fibrinogen into insoluble fibrin strands. This product, Thrombin CAS NO 62046-50-8, is essential for achieving rapid and reliable hemostasis in both clinical and research settings. It is a vital reagent for pharmaceutical manufacturers, diagnostic kit producers, and life science research laboratories focused on coagulation studies, wound healing, and tissue engineering.

Application

• Hemostatic Agent Production: A key active ingredient in topical hemostats, sealants, and glues used in surgical procedures to control bleeding.
• In Vitro Diagnostic (IVD) Reagents: Used as a critical component in coagulation analyzers and test kits to measure clotting times and assess coagulation factor function.
• Biomedical Research: Essential for studying blood coagulation mechanisms, platelet activation, and vascular biology in laboratory settings.
• Tissue Engineering & Bioprinting: Employed to cross-link fibrin-based bioinks and scaffolds for creating 3D tissue constructs.
• Cell Culture & Biochemistry: Used to harvest cells from culture vessels by cleaving cell-surface proteins and in various proteolytic assays.
• Food Processing: Utilized in some applications for blood fraction recovery and protein isolation in the meat industry.

Basic Information

Product Name	Thrombin
CAS No.	62046-50-8
Molecular Formula	Contact for details
Molecular Weight	~36,000 Da (approx., varies by source and isoform)
Synonyms	Thrombin, Bovine; Fibrinogenase; Activated Blood-Coagulation Factor II; Coagulation Factor IIa; E.C. 3.4.21.5; Thrombin Topical (Bovine); Thrombosin
EINECS	Contact for details

Quality Control

Our Thrombin is manufactured and tested under strict quality systems to ensure it meets the high standards required for pharmaceutical and diagnostic applications. Each batch is rigorously analyzed for specific activity, purity, and the absence of contaminating proteases. Certificates of Analysis (COA) detailing specifications such as activity (NIH units/mg), protein content, and sterility (where applicable) are available upon request.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store lyophilized powder at 2-8°C. Once reconstituted, aliquot and store at -20°C or below to prevent autolysis and loss of activity. The product is hygroscopic (moisture-sensitive); ensure containers are sealed tightly after use to prevent moisture absorption.

Specification

Item	Specification
Appearance	White to off-white lyophilized powder
Identification (IR)	Conforms
Specific Activity	≥ 1000 NIH units/mg protein
Protein Content (Biuret)	60 - 80 %
Solubility	Clear, colorless solution in water or saline
Loss on Drying	≤ 5.0 %
Bacterial Endotoxins (LAL)	< 0.1 EU/NIH unit

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.