Description

Ala-Ala-Phe-Mca Hydrochloride is a specialized fluorogenic peptide substrate designed for sensitive protease activity assays. This compound is critical for researchers and developers requiring precise, high-throughput enzymatic analysis in biochemical and pharmaceutical applications. It is primarily utilized in life science research, drug discovery, and diagnostic kit development for studying enzyme kinetics and inhibitor screening.

Application

• Protease Research: As a specific fluorogenic substrate for studying the activity and kinetics of various proteolytic enzymes.
• Drug Discovery & Screening: High-throughput screening (HTS) of potential protease inhibitors in pharmaceutical development pipelines.
• Biochemical Assay Development: A key component in the formulation of sensitive diagnostic and research assay kits.
• Enzyme Characterization: Used to determine kinetic parameters (Km, Vmax, kcat) and specificity of proteases.
• Cell Biology Studies: Investigating protease involvement in cellular processes like apoptosis, signal transduction, and protein degradation.
• Quality Control in Biologics: Monitoring protease activity during the production and purification of biopharmaceuticals.

Basic Information

Product Name	Ala-Ala-Phe-Mca Hydrochloride
CAS No.	62037-41-6
Molecular Formula	C30H37N5O7 • HCl
Molecular Weight	624.11 g/mol
Synonyms	Ala-Ala-Phe-AMC HCl; AAF-MCA Hydrochloride; L-Alanyl-L-alanyl-L-phenylalanyl-7-amido-4-methylcoumarin Hydrochloride; Fluorogenic Substrate AAF-MCA; 7-Amido-4-methylcoumarin conjugate of Ala-Ala-Phe; AAF-AMC HCl; Protease Substrate V; Chymotrypsin Substrate AAF-MCA
EINECS	Contact for details

Quality Control

Our Ala-Ala-Phe-Mca Hydrochloride is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity, identity confirmation by spectroscopic methods, and verification of fluorogenic activity. Certificates of Analysis (COA) detailing all specifications are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture absorption.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC/MS)	Conforms
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 5.0%
Fluorogenic Activity	Functional
Solubility	Soluble in DMSO, DMF; Slightly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.