Description

4-Nitrophenyl2-Acetamido-3,6-Di-o-Acety-2-Deoxyl-B-D-Glucopyranoside is a specialized glycoside derivative that serves as a critical chromogenic substrate for enzymatic assays. This compound matters for its high specificity and sensitivity in detecting and quantifying the activity of glycoside hydrolases, particularly β-N-acetylglucosaminidases. It is primarily needed by research institutions, diagnostic kit manufacturers, and pharmaceutical companies engaged in biochemical research, enzyme characterization, and the development of diagnostic tools for lysosomal storage disorders.

Application

• Enzyme Substrate for Biochemical Research: Used as a chromogenic substrate for the spectrophotometric assay of β-N-acetylglucosaminidase (NAGase) activity.
• Diagnostic Reagent Manufacturing: A key raw material in the production of diagnostic kits for measuring NAGase levels, a biomarker for renal tubular damage and certain genetic disorders.
• Lysosomal Storage Disorder Studies: Employed in research focused on enzymes involved in glycosaminoglycan degradation, relevant to conditions like Tay-Sachs and Sandhoff diseases.
• Microbiology & Cell Biology: Useful in studies of bacterial and fungal cell wall hydrolases and in tracking enzyme activity within cellular compartments.
• Pharmaceutical Development: Utilized in high-throughput screening (HTS) assays to identify potential inhibitors or activators of specific glycosidases for therapeutic development.
• Quality Control in Enzyme Production: Serves as a standard reagent for the purity and activity assessment of commercially produced glycosidase enzymes.

Basic Information

Product Name	4-Nitrophenyl2-Acetamido-3,6-Di-o-Acety-2-Deoxyl-B-D-Glucopyranoside
CAS No.	61891-87-0
Molecular Formula	C20H24N2O11
Molecular Weight	468.41 g/mol
Synonyms	4-Nitrophenyl N-acetyl-β-D-glucosaminide, di-O-acetylated; p-Nitrophenyl 2-acetamido-3,6-di-O-acetyl-2-deoxy-β-D-glucopyranoside; PNP-NAG diacetate; 4-Nitrophenyl 2-(acetylamino)-2-deoxy-3,6-di-O-acetyl-β-D-glucopyranoside; β-D-Glucopyranoside, 4-nitrophenyl 2-(acetylamino)-2-deoxy-3,6-di-O-acetyl-; p-Nitrophenyl β-D-N-acetylglucosaminide diacetate; NAGase Substrate, diacetylated; 61891-87-0
EINECS	Contact for details

Quality Control

Our 4-Nitrophenyl2-Acetamido-3,6-Di-o-Acety-2-Deoxyl-B-D-Glucopyranoside is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation via IR and NMR, to ensure it meets the high standards required for research and diagnostic applications. A comprehensive Certificate of Analysis (COA) detailing purity, assay, and impurity profiles is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C. This product is hygroscopic (moisture-sensitive) and light-sensitive; prolonged exposure to ambient humidity or light may affect stability and performance. The storage area should be well-ventilated and away from incompatible materials.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Identification (HPLC)	Retention time matches reference standard
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Residue on Ignition	≤ 0.1%
Specific Rotation	Contact for details
Solubility	Soluble in DMSO, DMF; sparingly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.