Description

Luciferase is a vital enzyme that catalyzes the light-emitting reaction in bioluminescence, serving as a cornerstone technology in modern life science research. Its primary value lies in providing an exceptionally sensitive, non-invasive, and quantitative reporter system for monitoring biological processes in real-time. This product is essential for researchers and developers in the pharmaceutical, biotechnology, and academic sectors who require reliable, high-activity enzyme preparations for advanced assay development.

Application

• Reporter Gene Assays: Widely used as a standard reporter for studying gene expression, promoter activity, and transcriptional regulation in cell-based and in vivo models.
• High-Throughput Screening (HTS): Enables rapid and sensitive detection in drug discovery campaigns for identifying potential therapeutic compounds that modulate specific biological pathways.
• Bioluminescence Imaging (BLI): Critical for non-invasive, longitudinal tracking of tumor growth, metastasis, infection progression, and cell trafficking in small animal studies.
• ATP Detection & Cell Viability Assays: Forms the basis of highly sensitive kits for measuring cellular ATP levels, a direct indicator of cell viability and metabolic activity.
• Protein-Protein Interaction Studies: Utilized in techniques like Bioluminescence Resonance Energy Transfer (BRET) to investigate molecular interactions in live cells.
• Environmental & Food Safety Monitoring: Employed in biosensor development for the detection of specific contaminants, pathogens, or toxic compounds.

Basic Information

Product Name	Luciferase
CAS No.	61869-41-8
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	Firefly Luciferase; Photinus-luciferin 4-monooxygenase (ATP-hydrolyzing); Beetle Luciferase; Luciferase (Photinus pyralis); D-Luciferin 4-monooxygenase; ATP-dependent Luciferase; EC 1.13.12.7
EINECS	Contact for details

Quality Control

Our Luciferase is produced and tested under stringent quality management systems to ensure batch-to-batch consistency and optimal performance. Each lot undergoes rigorous analytical testing, including activity assays, purity assessment, and stability checks. A comprehensive Certificate of Analysis (COA) detailing specific activity, purity, and functional performance is provided with every shipment to support your research and regulatory documentation needs.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below for long-term stability. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature before opening to minimize moisture condensation. For prolonged storage of reconstituted or diluted solutions, consult the specific technical data sheet.

Specification

Item	Specification
Appearance	Lyophilized powder or solution
Specific Activity	≥ 1 x 10^7 RLU/µg (typical)
Purity (SDS-PAGE)	≥ 90%
Identification (Activity)	Positive bioluminescence reaction with D-Luciferin and ATP
Endotoxin Level	< 1.0 EU/µg (for in vivo grade)
Solubility	Soluble in appropriate aqueous buffers

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.