Description

12-α-Hydroxysteroid Dehydrogenase is a highly specific oxidoreductase enzyme critical for the biosynthesis and metabolism of steroid hormones. This biocatalyst is essential for researchers and manufacturers requiring precise stereochemical control in steroid transformations. It is primarily utilized in the pharmaceutical, biotechnology, and academic research sectors for the development of steroid-based therapeutics, diagnostic assays, and metabolic pathway studies.

Application

• Pharmaceutical Intermediate Synthesis: Key biocatalyst for the selective 12-α-hydroxylation step in the production of bile acid analogs and specific corticosteroid precursors.
• Biocatalysis & Process Development: Used in green chemistry applications for the stereospecific modification of steroid cores, offering an alternative to traditional chemical synthesis.
• Diagnostic Reagent Manufacturing: A critical component in enzymatic assay kits for clinical and research laboratories to quantify specific steroid metabolites or enzyme activity.
• Metabolic Pathway Research: Employed in biochemical studies to investigate steroid hormone biosynthesis, degradation, and related metabolic disorders.
• Enzyme Engineering & Immobilization: Serves as a model enzyme for protein engineering projects aimed at improving stability, activity, or substrate specificity for industrial applications.

Basic Information

Product Name	12-α-Hydroxysteroid Dehydrogenase
CAS No.	61642-40-8
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	12α-HSD; 12α-Hydroxysteroid Dehydrogenase; 12-α-HSD; EC 1.1.1.176; Steroid 12-α-dehydrogenase; 12α-Hydroxysteroid:NAD(P)+ 12-oxidoreductase
EINECS	Contact for details

Quality Control

Our 12-α-Hydroxysteroid Dehydrogenase is produced and tested under a rigorous quality management system. Each lot is characterized for specific activity, purity, and stability to ensure batch-to-batch consistency for your critical applications. A comprehensive Certificate of Analysis (COA) detailing purity, activity units, and stability data is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store at -20°C or below. This product is hygroscopic (moisture-sensitive); allow the vial to equilibrate to room temperature in a dry environment before opening to prevent condensation and moisture uptake.

Specification

Item	Specification
Appearance	Lyophilized powder or solution
Identification (Activity)	Conforms
Specific Activity	≥ [Value] units/mg protein
Purity (SDS-PAGE)	≥ 90%
Protein Content (Bradford)	Reported per batch
Stability	Retains ≥ 90% activity after specified storage period
Endotoxin Level	< 1.0 EU/μg (for research-grade)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.