Description

Ac-Ala-Pro-Ala-Pna is a specialized peptide substrate designed for enzymatic activity studies. This compound is critical for researchers and quality control laboratories requiring precise, reliable tools for protease and peptidase assays. It is primarily utilized in biochemical research, pharmaceutical development, and diagnostic applications where the measurement of specific enzymatic activity is paramount.

Application

• Biochemical Research: Used as a specific chromogenic substrate for studying the kinetics and inhibition of proteolytic enzymes like dipeptidyl peptidase IV (DPP-IV).
• Pharmaceutical Screening: Employed in high-throughput screening (HTS) assays to identify and characterize potential drug candidates targeting protease activity.
• Diagnostic Reagent Manufacturing: Serves as a key component in the formulation of diagnostic kits for clinical analysis of enzyme levels.
• Enzyme Characterization: Essential for determining the substrate specificity and catalytic efficiency of newly discovered or engineered proteases.
• Quality Control (QC): Applied in biopharmaceutical QC labs to monitor the activity and consistency of enzyme-based products during manufacturing.

Basic Information

Product Name	Ac-Ala-Pro-Ala-Pna
CAS No.	61596-39-2
Molecular Formula	C21H28N6O6
Molecular Weight	460.49 g/mol
Synonyms	N-Acetyl-Ala-Pro-Ala-p-nitroanilide; Acetyl-Alanyl-Prolyl-Alanyl-p-nitroanilide; Ac-Ala-Pro-Ala-pNA; DPP-IV Substrate; H-Ala-Pro-Ala-pNA, N-Acetyl-; Ala-Pro-Ala-pNA, N-Acetyl-; 61596-39-2; Acetylalanylprolylalanyl-4-nitroanilide
EINECS	Contact for details

Quality Control

Our Ac-Ala-Pro-Ala-Pna is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity verification and identity confirmation by mass spectrometry (MS) and infrared spectroscopy (IR). Certificates of Analysis (COA) detailing all specifications are provided to ensure traceability and compliance with your research or production standards.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms
Identification (MS)	Conforms
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 5.0%
Solubility	Soluble in DMSO, DMF; slightly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.