Description

n-Succinyl-Gly-Gly-Gly p-Nitroanilide is a synthetic tripeptide derivative designed as a chromogenic substrate for the study of proteolytic enzyme activity. This compound matters for its high specificity and sensitivity in biochemical assays, enabling precise kinetic measurements and inhibitor screening. It is primarily needed by research institutions, pharmaceutical R&D laboratories, and diagnostic kit manufacturers focused on enzymology and protease research.

Application

• Protease/Peptidase Substrate: A chromogenic substrate for the kinetic assay and characterization of various serine and cysteine proteases.
• Biochemical Research: Used in fundamental enzymology studies to determine enzyme kinetics, inhibition constants (Ki), and catalytic mechanisms.
• Pharmaceutical Screening: Employed in high-throughput screening (HTS) platforms to identify and evaluate potential protease inhibitors for drug discovery.
• Diagnostic Development: Serves as a key reagent in developing clinical diagnostic assays for measuring specific protease activity in biological samples.
• Quality Control in Biotechnology: Used to assess the activity and consistency of enzyme preparations in biomanufacturing processes.

Basic Information

Product Name	n-Succinyl-Gly-Gly-Gly p-Nitroanilide
CAS No.	61043-71-8
Molecular Formula	C₁₇H₂₁N₅O₈
Molecular Weight	423.38 g/mol
Synonyms	Succinyl-Gly-Gly-Gly p-Nitroanilide; Suc-Gly-Gly-Gly-pNA; N-Succinylglycylglycylglycine 4-nitroanilide; Suc-GGG-pNA; Protease Substrate Suc-GGG-pNA; 4-Nitroanilide of N-Succinyl-triglycine; N-Succinyl-L-glycyl-L-glycyl-L-glycine p-nitroanilide
EINECS	Contact for details

Quality Control

Our n-Succinyl-Gly-Gly-Gly p-Nitroanilide is manufactured under strict quality management systems to ensure high purity and batch-to-batch consistency, suitable for sensitive research applications. Each lot undergoes comprehensive analytical testing including HPLC for purity, identity confirmation by IR and NMR, and verification of performance in functional assays. Certificates of Analysis (COA) with detailed specifications are provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 15-25°C. The product is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use to prevent absorption of atmospheric moisture, which may affect stability and performance.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Residue on Ignition	≤ 0.5%
Functional Activity	Conforms to reference standard

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.