Description

Succinyl-Alanyl-Alanyl-Phenylalanine-4-Nitroanilide is a high-purity, chromogenic peptide substrate specifically designed for enzymatic research and diagnostic applications. This compound is critical for the precise measurement of protease activity, particularly elastase, due to its specific cleavage site. It is an essential reagent for researchers and manufacturers in the pharmaceutical, biotechnology, and clinical diagnostics sectors who require reliable and sensitive tools for enzyme kinetics studies and assay development.

Application

• Protease Activity Assays: Primary substrate for the colorimetric determination of human leukocyte elastase (HLE) and related serine proteases.
• Drug Discovery & Development: Used in high-throughput screening (HTS) platforms to identify and characterize novel elastase inhibitors for therapeutic targets.
• Diagnostic Kit Manufacturing: A key component in the formulation of clinical diagnostic kits for measuring elastase levels in biological samples.
• Biochemical Research: Employed in academic and industrial laboratories for fundamental studies on enzyme mechanisms, kinetics, and specificity.
• Quality Control in Biologics: Used to monitor protease activity during the production and purification of biopharmaceuticals.

Basic Information

Product Name	Succinyl-Alanyl-Alanyl-Phenylalanine-4-Nitroanilide
CAS No.	61043-53-6
Molecular Formula	C26H31N7O8
Molecular Weight	569.57 g/mol
Synonyms	Suc-AAPF-pNA; N-Succinyl-Ala-Ala-Pro-Phe-p-nitroanilide; Suc-Ala-Ala-Pro-Phe-pNA; Elastase Substrate IV; N-Succinyl-L-alanyl-L-alanyl-L-prolyl-L-phenylalanine 4-nitroanilide; AAPF-pNA; Suc-AAPF-pNA Substrate; Human Leukocyte Elastase Substrate
EINECS	Contact for details

Quality Control

Our Succinyl-Alanyl-Alanyl-Phenylalanine-4-Nitroanilide is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity, identity confirmation by IR and MS, and specific activity validation to ensure performance consistency. Certificates of Analysis (COA) with detailed chromatographic data are provided to guarantee compliance with your research and production standards.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and hydrolysis.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Identification (HPLC)	Conforms to reference standard
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Specific Optical Rotation	Contact for details
Solubility	Soluble in DMSO, DMF; slightly soluble in water
Assay (Enzymatic)	≥ 95% of theoretical activity

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.