Description

Ala-Ala-Phe p-Nitroanilide is a specialized tripeptide derivative chromogenic substrate designed for enzymatic assays. This compound is of critical importance in biochemical research and pharmaceutical development for its role in measuring protease and peptidase activity. It is primarily needed by research institutions, diagnostic kit manufacturers, and pharmaceutical companies engaged in enzyme kinetics studies and high-throughput screening.

Application

• Protease & Peptidase Research: As a chromogenic substrate for the kinetic study and characterization of various proteolytic enzymes, including chymotrypsin, subtilisin, and other serine proteases.
• Diagnostic Reagent Manufacturing: A key component in the formulation of diagnostic kits for detecting and quantifying specific enzyme activities in clinical and research samples.
• Pharmaceutical Screening: Used in high-throughput screening (HTS) assays to identify and evaluate potential protease inhibitors for drug discovery programs.
• Biochemical Assay Development: Employed in the development and validation of new enzymatic assay protocols in academic and industrial laboratories.
• Quality Control in Biologics: For monitoring protease activity during the production and purification of protein-based therapeutics to ensure product consistency and safety.

Basic Information

Product Name	Ala-Ala-Phe p-Nitroanilide
CAS No.	61043-41-2
Molecular Formula	C21H25N5O6
Molecular Weight	443.46 g/mol
Synonyms	L-Alanyl-L-alanyl-L-phenylalanine 4-nitroanilide; AAF-pNA; Ala-Ala-Phe-pNA; N-Succinyl-Ala-Ala-Phe-p-nitroanilide (related derivative); Protease Substrate AAF-pNA; Chymotrypsin Substrate; Suc-AAPF-pNA (commonly associated); Tripeptide p-nitroanilide substrate
EINECS	Contact for details

Quality Control

Our Ala-Ala-Phe p-Nitroanilide is produced under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity verification and identity confirmation by spectroscopic methods, to ensure it meets the high standards required for sensitive biochemical applications. Certificates of Analysis (COA) with detailed chromatographic data are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 15-25°C. This product is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use to prevent degradation from atmospheric moisture.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF; sparingly soluble in water
Specific Rotation	Contact for details

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.