Description

Z-Ala-Ala-Leu-Pna is a synthetic peptide substrate designed for enzymatic activity studies. This compound is a critical tool for researchers and quality control laboratories requiring precise measurement of protease and peptidase activity. It is primarily utilized in the pharmaceutical, biotechnology, and biochemical research sectors for drug discovery, enzyme characterization, and diagnostic assay development.

Application

• Protease/Peptidase Assays: As a chromogenic substrate for the kinetic analysis and inhibition studies of serine and cysteine proteases.
• Pharmaceutical Research: High-throughput screening (HTS) for identifying and characterizing novel protease inhibitors in drug discovery pipelines.
• Biochemical Research: Enzyme mechanism studies and determination of kinetic parameters (Km, Vmax, kcat).
• Diagnostic Development: A key component in the formulation of diagnostic kits for detecting specific protease activities related to diseases.
• Quality Control: Used in biomanufacturing to monitor and validate the activity of enzyme-based products and processes.

Basic Information

Product Name	Z-Ala-Ala-Leu-Pna
CAS No.	61043-33-2
Molecular Formula	C24H30N4O6
Molecular Weight	470.52 g/mol
Synonyms	Z-Ala-Ala-Leu-pNA; N-CBZ-Ala-Ala-Leu-p-Nitroanilide; N-Benzyloxycarbonyl-Ala-Ala-Leu-p-Nitroanilide; Ala-Ala-Leu-pNA (Z-); CBZ-Ala-Ala-Leu-pNA; Substrate for Cathepsin B; Cathepsin B Substrate; Protease Substrate Z-AAL-pNA
EINECS	Contact for details

Quality Control

Our Z-Ala-Ala-Leu-Pna is produced under strict quality management systems to ensure batch-to-batch consistency and high purity for research applications. Each lot undergoes comprehensive analytical testing including HPLC for purity, NMR for structural confirmation, and mass spectrometry. A Certificate of Analysis (COA) detailing all specifications and test results is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms
Identification (MS)	Conforms
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF; slightly soluble in methanol

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.