Description

8-Azidoadenosine 5'-Monophosphate is a chemically modified nucleotide analog where an azido group replaces the hydroxyl group at the 8-position of the adenine ring. This modification makes it a valuable photoaffinity labeling reagent and a versatile chemical probe for studying nucleotide-binding proteins and enzymes. It is primarily utilized by researchers in biochemistry, molecular biology, and medicinal chemistry for investigating enzyme mechanisms, protein-ligand interactions, and for the development of novel diagnostic and therapeutic agents.

Application

• Photoaffinity Labeling: Acts as a critical cross-linking agent to covalently tag and identify nucleotide-binding sites on proteins and enzymes upon UV irradiation.
• Enzyme Mechanism Studies: Used as an inhibitor or substrate analog to probe the active sites and catalytic mechanisms of kinases, ATPases, and other adenylate-utilizing enzymes.
• Bioconjugation & Probe Synthesis: Serves as a key intermediate for synthesizing fluorescent, biotinylated, or other functionalized nucleotide probes via click chemistry (azide-alkyne cycloaddition).
• Structural Biology: Aids in X-ray crystallography and NMR studies by providing a modified ligand to solve protein-nucleotide complex structures.
• Drug Discovery Research: Employed in high-throughput screening and target validation for diseases involving purinergic signaling pathways.
• Diagnostic Assay Development: Used in the design of sensitive assays for detecting specific enzymatic activities or protein biomarkers.

Basic Information

Item	Details
Product Name	8-Azidoadenosine 5'-Monophosphate
CAS No.	60731-47-7
Molecular Formula	C10H12N8O7P
Molecular Weight	378.22 g/mol
Synonyms	8-Azido-AMP; 8-Azidoadenosine monophosphate; 8-N3-AMP; 8-Azido-5'-adenylic acid; 8-Azidoadenosine 5'-(dihydrogen phosphate); Adenosine-8-azido-5'-monophosphate; 5'-O-Phosphono-8-azidoadenosine
EINECS	Contact for details

Quality Control

Our 8-Azidoadenosine 5'-Monophosphate is produced under stringent quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity, NMR and MS for structural confirmation, and verification of the azide functionality. Certificates of Analysis (COA) detailing purity, identity, and impurity profiles are provided to ensure the material meets the exacting standards required for research and development applications.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This compound is hygroscopic (moisture-sensitive) and light-sensitive. For long-term storage, keep under an inert atmosphere. Allow the container to reach room temperature before opening to minimize moisture condensation.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Retention time corresponds to reference standard
Identification (NMR)	Spectrum consistent with structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 3.0%
Solubility	Soluble in water, DMSO

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.