Description

5'-Phosphodiesterase is a crucial enzyme that catalyzes the hydrolysis of phosphodiester bonds in nucleic acids, specifically targeting the 5' position. This biochemical activity makes it an essential tool for research and development in molecular biology and enzymology. It is primarily utilized by biotechnology firms, pharmaceutical research laboratories, and academic institutions for applications in nucleic acid analysis, sequencing, and the study of nucleotide metabolism.

Application

• Nucleic Acid Research: Used for the controlled hydrolysis of RNA and DNA to study structure, sequence, and function.
• Biochemical Assay Development: Serves as a key component in enzymatic assays to measure phosphodiesterase activity or screen for inhibitors.
• Pharmaceutical R&D: Employed in drug discovery programs targeting nucleotide-based pathways, including research into antiviral and anticancer agents.
• Molecular Biology Tool: Facilitates the generation of specific nucleic acid fragments for cloning, mapping, and other downstream analytical techniques.
• Enzymology Studies: Used as a model enzyme to investigate reaction mechanisms, kinetics, and the properties of phosphodiesterases.
• Diagnostic Kit Manufacturing: Incorporated as a reagent in diagnostic kits for the detection and quantification of specific nucleic acid sequences.

Basic Information

Product Name	5'-Phosphodiesterase
CAS No.	60675-83-4
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	5'-PDE; PDE I; Venom Exonuclease; Snake Venom Phosphodiesterase; Oligonucleate 5'-nucleotidohydrolase; EC 3.1.4.1; Phosphodiesterase I; 5'-Exonuclease
EINECS	Contact for details

Quality Control

Our 5'-Phosphodiesterase is produced and tested under stringent quality management systems to ensure batch-to-batch consistency and high enzymatic activity. Each lot is characterized for specific activity, purity, and absence of contaminating nucleases. A comprehensive Certificate of Analysis (COA) detailing assay results, stability data, and storage conditions is provided with every shipment to support your quality assurance protocols.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store at -20°C or below. Avoid repeated freeze-thaw cycles to maintain optimal enzymatic activity. The product is hygroscopic (moisture-sensitive).

Specification

Item	Specification
Appearance	White to off-white lyophilized powder
Identification (IR)	Conforms to standard
Specific Activity	≥ [Value] units/mg protein
Protein Content (Biuret)	≥ [Value] %
Purity (SDS-PAGE)	≥ [Value] %
Endonuclease Activity	Not detected
RNase Activity	Not detected
Solubility	Clear, colorless solution in specified buffer
pH (in solution)	[Value]

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.