Description

Guanosine, 5'-(Trihydrogen Imidodiphosphate), Tetralithium Salt is a high-purity biochemical reagent, specifically a nucleotide analog. This compound is valued for its role as a stable, non-hydrolyzable analog of GTP (guanosine triphosphate), making it a critical tool for studying GTP-binding proteins and nucleotide-dependent enzymatic processes. It is essential for researchers and manufacturers in the fields of molecular biology, enzymology, and pharmaceutical development, where precise control over nucleotide signaling pathways is required.

Application

• Biochemical Research: Used as a non-hydrolyzable GTP analog (GppNHp) to study the activation and regulation of G-proteins, GTPases, and other nucleotide-binding proteins.
• Enzyme Kinetics & Inhibition Studies: Serves as a substrate analog to investigate enzyme mechanisms, particularly those involving kinases, polymerases, and ATP/GTP-dependent enzymes.
• Pharmaceutical Screening: Employed in high-throughput screening (HTS) assays to identify potential drug candidates that target GTPase signaling pathways, relevant in oncology and neurology.
• Diagnostic Reagent Manufacturing: A key component in the formulation of diagnostic kits for research and clinical analysis of cellular signaling and metabolic disorders.
• Structural Biology: Utilized in X-ray crystallography and NMR studies to determine the three-dimensional structure of protein-nucleotide complexes.
• Cell Signaling & Physiology Studies: Applied in cell-based assays to probe the role of GTP in processes like protein synthesis, signal transduction, and cytoskeletal dynamics.

Basic Information

Product Name	Guanosine, 5'-(Trihydrogen Imidodiphosphate), Tetralithium Salt
CAS No.	60522-21-6
Molecular Formula	C10H14Li4N6O13P2
Molecular Weight	556.04 g/mol
Synonyms	Guanosine 5'-[β,γ-imido]triphosphate tetralithium salt; GppNHp tetralithium salt; Guanosine-5'-[β,γ-imido]triphosphate, Li4 salt; 5'-Guanylyl imidodiphosphate tetralithium salt; GMP-PNP tetralithium salt; GTPγS analog; Non-hydrolyzable GTP analog; 5'-Guanosine triphosphate, imido, tetralithium salt
EINECS	Contact for details

Quality Control

Our Guanosine, 5'-(Trihydrogen Imidodiphosphate), Tetralithium Salt is manufactured under strict quality systems to ensure batch-to-batch consistency and high purity suitable for sensitive research applications. All products undergo rigorous analytical testing, including HPLC, NMR, and mass spectrometry, to confirm identity and purity. A comprehensive Certificate of Analysis (COA) detailing purity, impurities, and analytical methods is provided with each shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture absorption.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms
Identification (NMR)	Conforms
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 5.0%
pH (1% Solution)	6.0 - 8.0
Endotoxin (LAL)	< 10 EU/mg
Biological Activity	Tested in standard G-protein binding assay

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.