Description

Adenosine 5'-o-(2-Thiotriphosphate) is a chemically modified nucleotide analog where a sulfur atom replaces one of the non-bridging oxygen atoms in the triphosphate chain. This thiophosphate modification makes it a crucial tool in biochemical research, particularly as a substrate for kinases and other ATP-dependent enzymes to study reaction mechanisms and probe enzyme active sites. It is essential for researchers in molecular biology, enzymology, and drug discovery who require high-purity, well-characterized biochemical reagents for sensitive assays and structural studies.

Application

• Enzyme Kinetics & Mechanism Studies: Used as a substrate analog to investigate the catalytic mechanisms of ATP-dependent enzymes like kinases, polymerases, and helicases.
• Phosphorylation Assays: Serves as a thiophosphate donor in in vitro phosphorylation experiments, allowing for subsequent tracking or isolation of thiophosphorylated products.
• Structural Biology: Aids in X-ray crystallography and NMR studies to capture enzyme-substrate complexes and understand binding interactions.
• Drug Discovery & Screening: Employed in high-throughput screening assays to identify and characterize inhibitors of kinase and other nucleotide-binding targets.
• Biochemical Probe Synthesis: Acts as a building block for synthesizing more complex probes, such as fluorescently labeled or biotinylated ATP analogs.
• Signal Transduction Research: Utilized to study ATP-dependent processes in cell signaling pathways under controlled experimental conditions.

Basic Information

Product Name	Adenosine 5'-o-(2-Thiotriphosphate)
CAS No.	60478-94-6
Molecular Formula	C10H16N5O12P3S
Molecular Weight	523.31 g/mol
Synonyms	Adenosine 5'-(γ-thio)triphosphate; ATPγS; Adenosine 5'-[γ-thio]triphosphate; 2'-Deoxyadenosine-5'-(1-thiotriphosphate); γ-Thio-ATP; Adenosine 5'-triphosphate, 1-thio-; 5'-ATP-γ-S; Guanosine 5'-[γ-thio]triphosphate (common related analog)
EINECS	Contact for details

Quality Control

Our Adenosine 5'-o-(2-Thiotriphosphate) is manufactured under stringent conditions to ensure the highest standards of purity and consistency, suitable for sensitive research applications. Each batch is supported by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and impurity profiles. We adhere to ISO 9001 quality management principles, and our production processes are designed to meet the exacting requirements of the life sciences research community.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive). For long-term storage, consider desiccant and under an inert atmosphere to maintain stability and potency. Allow the product to equilibrate to room temperature before opening to minimize moisture condensation.

Specification

Item	Specification
Appearance	White to off-white powder or solid
Identification (HPLC)	Conforms
Identification (NMR)	Conforms
Purity (HPLC)	≥ 95%
Assay (Enzymatic)	≥ 90%
Water Content (KF)	≤ 3.0%
Solubility	Soluble in water or buffer

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.