Description

n-Benzoyl-Ile-Glu-Gly-Arg p-*Nitroanilide Hydrochlor CAS NO 60457-00-3 is a specialized chromogenic peptide substrate designed for the selective detection and quantification of specific protease activity. This compound is critical for researchers and quality control laboratories requiring precise, reproducible enzymatic assays. It is primarily utilized in biochemical research, pharmaceutical development, and diagnostic kit manufacturing for studying coagulation factors and other serine proteases.

Application

• Biochemical Research: Used as a substrate in kinetic studies to determine the activity and inhibition of target proteases like thrombin, factor Xa, and other trypsin-like enzymes.
• Pharmaceutical Development: Employed in high-throughput screening (HTS) assays to identify and characterize novel protease inhibitors for drug discovery programs.
• Diagnostic Manufacturing: A key component in the formulation of chromogenic assay kits for clinical diagnostics, particularly for hemostasis testing and coagulation factor analysis.
• Quality Control (QC): Serves as a reference standard in QC laboratories for batch testing and validating the potency of therapeutic enzymes or related biopharmaceuticals.
• Enzyme Characterization: Facilitates the determination of kinetic parameters (Km, Vmax) and specificity constants for purified enzymes.

Basic Information

Product Name	n-Benzoyl-Ile-Glu-Gly-Arg p-*Nitroanilide Hydrochlor
CAS No.	60457-00-3
Molecular Formula	C31H41N9O9 • HCl
Molecular Weight	Contact for details
Synonyms	Bz-Ile-Glu-Gly-Arg-pNA • HCl; Benzoyl-Isoleucine-Glutamyl-Glycyl-Arginine p-Nitroanilide Hydrochloride; Chromogenic Substrate S-2238 (related analog); H-D-Ile-Glu-Gly-Arg-pNA • HCl (stereoisomer); Bz-Ile-Glu-Gly-Arg-p-nitroanilide hydrochloride; Protease Substrate 60457-00-3; Thrombin Chromogenic Substrate (type); Boc-IEGR-pNA (related analog)
EINECS	Contact for details

Quality Control

Our n-Benzoyl-Ile-Glu-Gly-Arg p-*Nitroanilide Hydrochlor is manufactured under strict quality systems to ensure batch-to-batch consistency and reliability for sensitive research applications. Each lot undergoes comprehensive analytical testing, including HPLC for purity and identity confirmation. A Certificate of Analysis (COA) detailing purity, water content, and performance in standard enzymatic assays is provided with every shipment to support your quality and regulatory requirements.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 5.0%
Assay (Enzymatic)	≥ 90% of theoretical activity
Solubility	Soluble in water, DMSO

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.