Description

(+/-)Amethopterin CAS NO 60388-53-6 is a racemic mixture of the potent antifolate agent, Methotrexate, which is a critical compound in biochemical and pharmaceutical research. This product is essential for researchers and developers studying the mechanisms of folate metabolism, enzyme inhibition, and the development of therapeutic analogs. It serves as a key reference standard and starting material for analytical laboratories, academic institutions, and pharmaceutical companies engaged in oncology and autoimmune disease research.

Application

• Pharmaceutical Reference Standard: Used for the calibration of analytical instruments and the validation of assay methods in quality control laboratories.
• Biochemical Research: Serves as a critical tool for studying dihydrofolate reductase (DHFR) inhibition and folate-dependent metabolic pathways.
• Drug Development: Acts as a precursor or a comparative compound in the synthesis and evaluation of novel antifolate and chemotherapeutic agents.
• Academic Studies: Utilized in university and institutional research for investigating cell proliferation, apoptosis, and mechanisms of drug resistance.
• Analytical Method Development: Employed to develop and optimize chromatographic (HPLC, LC-MS) methods for the detection and quantification of related substances.

Basic Information

Product Name	(+/-)Amethopterin
CAS No.	60388-53-6
Molecular Formula	C₂₀H₂₂N₈O₅
Molecular Weight	454.44 g/mol
Synonyms	DL-Methotrexate; Racemic Methotrexate; (±)-Methotrexate; Amethopterin Racemate; NSC-740; L-Glutamic acid, N-[4-[[(2,4-diamino-6-pteridinyl)methyl]methylamino]benzoyl]-; (±)-N-[p-[[(2,4-Diamino-6-pteridinyl)methyl]methylamino]benzoyl]glutamic acid
EINECS	Contact for details

Quality Control

Our (+/-)Amethopterin is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including identity confirmation, purity assay, and impurity profiling to ensure it meets the stringent requirements for research and development use. A detailed Certificate of Analysis (COA) is provided with every shipment, documenting compliance with our internal specifications.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (typically 15-25°C). The product is light-sensitive and should be handled under appropriate conditions to maintain stability.

Specification

Item	Specification
Appearance	Yellow to orange crystalline powder
Identification (IR)	Conforms to reference spectrum
Identification (HPLC)	Retention time corresponds to reference standard
Assay (HPLC)	≥ 98.0%
Related Substances (HPLC)	Total impurities ≤ 2.0%
Water Content (KF)	≤ 1.0%
Residue on Ignition	≤ 0.1%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.