Description

Cholesterol, [7-3H(N)] is a high-specific-activity, tritium-labeled derivative of cholesterol, a fundamental sterol essential for cell membrane structure and function. This radiolabeled compound is critical for advanced metabolic and pharmacokinetic research, enabling precise tracking and quantification in biological systems. It is primarily utilized by pharmaceutical R&D laboratories, academic research institutions, and biotechnology companies conducting tracer studies in lipid metabolism, drug discovery, and receptor binding assays.

Application

• Metabolic Pathway Tracing: Used as a radiotracer to study cholesterol biosynthesis, absorption, and turnover in vivo and in vitro.
• Drug Discovery & Development: Facilitates high-throughput screening and ADME (Absorption, Distribution, Metabolism, Excretion) studies for lipid-regulating pharmaceuticals.
• Membrane Biology Research: Investigates the role of cholesterol in membrane fluidity, lipid raft formation, and protein-lipid interactions.
• Receptor Binding Assays: Serves as a ligand in competitive binding studies for nuclear receptors and other cholesterol-binding proteins.
• Biochemical Standard: Acts as an internal standard in analytical methods such as LC-MS for the quantification of cholesterol and its metabolites.
• Diagnostic Research: Applied in the development of assays for studying dyslipidemias and related cardiovascular diseases.

Basic Information

Product Name	Cholesterol, [7-3H(N)]
CAS No.	60280-55-9
Molecular Formula	C₂₇H₄₄O * (³H)
Molecular Weight	~389.6 g/mol (unlabeled)
Synonyms	(3β)-Cholest-5-en-3-ol, [7-3H]; Tritiated Cholesterol; Cholesterol-7-3H; Cholesterol tritiated; 5-Cholesten-3β-ol, [7-3H]; [7(n)-3H]Cholesterol; Radiolabeled Cholesterol; 3β-Hydroxy-5-cholestene, [7-3H]
EINECS	Contact for details

Quality Control

Every batch of Cholesterol, [7-3H(N)] is manufactured and analyzed under strict quality systems to ensure exceptional radiochemical purity and specific activity. Our products undergo rigorous quality testing to ensure compliance with industry standards. Certificates of Analysis (COA) detailing specific activity, radiochemical purity (by HPLC), chemical purity, and solvent composition are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C or as specified on the label. Due to its highly volatile nature (tritium), appropriate containment and handling procedures for radioactive materials must be followed at all times. For long-term storage, keep the product in the original shielded container in a designated, well-ventilated area.

Specification

Item	Specification
Appearance	Clear, colorless to pale yellow solution
Specific Activity	10-50 Ci/mmol (Contact for exact value)
Radiochemical Purity (HPLC)	≥ 97%
Chemical Purity (HPLC)	≥ 95%
Concentration	1 mCi/mL in ethanol (or as specified)
Solvent	Ethanol, Toluene, or custom solvent available
Identification (IR/NMR)	Conforms to structure

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.