Description

Alanylproline-4-Nitroanilide is a specialized chromogenic peptide substrate designed for enzymatic activity assays. This compound is critical for researchers and quality control laboratories requiring precise, colorimetric detection of specific protease activity. It is primarily utilized in the pharmaceutical, biotechnology, and diagnostic industries for enzyme kinetics studies, inhibitor screening, and biochemical research.

Application

• Protease Activity Assays: Used as a selective substrate for the colorimetric determination of dipeptidyl peptidase (DPP) and related protease activities.
• Biochemical Research: Essential tool in enzyme kinetics studies to measure reaction rates and characterize enzyme mechanisms.
• Pharmaceutical Screening: Employed in high-throughput screening (HTS) platforms to identify and evaluate potential protease inhibitors for drug discovery.
• Diagnostic Reagent Manufacturing: Serves as a key component in the formulation of diagnostic kits for clinical and research laboratories.
• Quality Control (QC): Applied in QC testing for biopharmaceuticals to monitor and validate the activity of enzyme-based products.

Basic Information

Product Name	Alanylproline-4-Nitroanilide
CAS No.	60189-44-8
Molecular Formula	C17H21N5O5
Molecular Weight	375.38 g/mol
Synonyms	Ala-Pro-pNA; L-Alanyl-L-proline 4-nitroanilide; H-Ala-Pro-pNA; APNA; Alanyl-Proline p-Nitroanilide; DPP-IV Substrate; Gly-Pro-pNA analog; Dipeptidyl Peptidase Substrate
EINECS	Contact for details

Quality Control

Our Alanylproline-4-Nitroanilide is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation, to ensure it meets the high standards required for research and diagnostic applications. Certificates of Analysis (COA) with detailed chromatographic data are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C. This product is hygroscopic (moisture-sensitive) and should be handled under anhydrous conditions to maintain stability.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF; slightly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.