Description

Glycylproline 4-Nitroanilide is a specialized chromogenic substrate designed for enzymatic activity assays. This compound is critical for researchers and quality control laboratories requiring precise, reliable, and reproducible results in biochemical studies. It is primarily utilized in the pharmaceutical, diagnostic, and life science research sectors for the study and quantification of specific proteolytic enzymes, such as dipeptidyl peptidase IV (DPP-IV).

Application

• Enzymatic Substrate for DPP-IV: Used as a selective chromogenic substrate for the kinetic study and inhibition screening of dipeptidyl peptidase IV (DPP-IV/CD26), a key target in type 2 diabetes and cancer research.
• Biochemical Research: Essential for in vitro assays to determine enzyme activity, specificity, and kinetics in academic and industrial research laboratories.
• Pharmaceutical Development: Employed in high-throughput screening (HTS) platforms to identify and characterize potential therapeutic inhibitors for drug discovery programs.
• Diagnostic Reagent Manufacturing: Serves as a key component in the formulation of diagnostic kits and reagents for clinical testing and biomarker analysis.
• Quality Control (QC) Testing: Used in QC laboratories for the potency testing and standardization of enzyme preparations and related biopharmaceutical products.

Basic Information

Product Name	Glycylproline 4-Nitroanilide
CAS No.	60189-43-7
Molecular Formula	C17H21N5O5
Molecular Weight	375.38 g/mol
Synonyms	Gly-Pro-pNA; H-Gly-Pro-p-nitroanilide; Glycyl-L-prolyl 4-nitroanilide; L-Glycyl-L-proline 4-nitroanilide; GP-pNA; DPP-IV Substrate; Dipeptidyl Peptidase IV Substrate; 4-Nitroanilide of Glycylproline
EINECS	Contact for details

Quality Control

Every batch of Glycylproline 4-Nitroanilide is manufactured under strict quality management systems and undergoes comprehensive analytical testing to ensure identity, purity, and performance consistency. We provide Certificates of Analysis (COA) with each shipment, detailing key parameters such as assay purity, spectral identification, and impurity profiles. Our quality commitment supports applications requiring research-grade and diagnostic-grade materials.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 15-25°C. This material is hygroscopic (moisture-sensitive) and should be handled in a low-humidity environment to prevent degradation. For long-term stability, consider storage under desiccant.

Specification

Item	Specification
Appearance	Yellow to orange crystalline powder
Identification (IR)	Conforms to structure
Assay (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Melting Point	Contact for details
Solubility	Soluble in DMSO, DMF; slightly soluble in water
Heavy Metals	< 10 ppm

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.