Description

n-Ethylmaleimide-γ-Glutamyl-Cysteinyl-Glycine is a specialized biochemical reagent, a derivative of glutathione modified with an N-ethylmaleimide (NEM) moiety. This compound is critically important as a research tool for studying thiol biochemistry, specifically for the selective and irreversible alkylation of cysteine residues in peptides and proteins. It is primarily utilized by researchers and scientists in the fields of biochemistry, proteomics, and pharmaceutical development for investigating redox biology, enzyme mechanisms, and protein structure-function relationships.

Application

• Biochemical Research: A key reagent for the selective alkylation and blocking of free thiol (-SH) groups in peptides and proteins to study their function and structure.
• Proteomics & Mass Spectrometry: Used in sample preparation to stabilize cysteine-containing peptides, prevent disulfide scrambling, and improve analysis in LC-MS workflows.
• Enzyme Inhibition Studies: Employed to investigate enzymes with active-site cysteine residues, helping to elucidate catalytic mechanisms and design inhibitors.
• Pharmaceutical Development: Serves as a tool compound in drug discovery pipelines targeting thiol-dependent pathways and oxidative stress responses.
• Antioxidant Research: Used to probe the role of glutathione and related systems in cellular redox signaling and defense against oxidative damage.
• Bioconjugation & Protein Labeling: Acts as a reactive handle for site-specific modification of biomolecules due to its maleimide functional group.

Basic Information

Item	Detail
Product Name	n-Ethylmaleimide-γ-Glutamyl-Cysteinyl-Glycine
CAS No.	60189-42-6
Molecular Formula	C16H23N4O8S
Molecular Weight	431.44 g/mol
Synonyms	NEM-Glutathione; N-Ethylmaleimide Glutathione; S-(N-Ethylsuccinimido)glutathione; Glutathione N-ethylmaleimide derivative; γ-Glu-Cys(NEM)-Gly; NSC 334156; NEM-GSH; Glutathione-N-ethylmaleimide adduct
EINECS	Contact for details

Quality Control

Our n-Ethylmaleimide-γ-Glutamyl-Cysteinyl-Glycine is produced under strict quality management protocols. Each batch undergoes comprehensive analytical testing to ensure high purity and consistency, meeting the stringent requirements of research applications. Certificates of Analysis (COA) detailing purity, identity, and impurity profiles are available upon request to support your quality assurance and regulatory documentation.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C in a dry environment. This product is hygroscopic (moisture-sensitive) and light-sensitive (store away from light). For long-term stability, consider storage under inert atmosphere or desiccant.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC/MS)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 5.0%
Heavy Metals	≤ 20 ppm
Residual Solvents (GC)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.