Description

p-Nitrophenyl β-D-Xylobioside is a specialized chromogenic substrate designed for the detection and quantification of xylanase enzyme activity. This compound matters because it provides a highly sensitive and convenient method for researchers and quality control labs to measure enzymatic hydrolysis rates, offering a clear colorimetric readout. It is primarily needed by the biotechnology, biofuel research, and pulp & paper industries for enzyme characterization, screening, and process optimization.

Application

• Xylanase Activity Assays: Primary substrate for the spectrophotometric determination of endo-1,4-β-xylanase activity in research and industrial samples.
• Enzyme Kinetics Studies: Used to study the mechanism, inhibition, and kinetic parameters (Km, Vmax) of xylanolytic enzymes.
• Biomass Conversion Research: A critical tool in biofuel and biorefinery research for evaluating the efficiency of hemicellulose-degrading enzyme cocktails.
• Pulp & Paper Industry QC: Employed in quality control to monitor xylanase enzyme preparations used in bio-bleaching processes.
• Microbiological & Agricultural Research: Used to assay xylanase production by microorganisms or to screen for novel enzyme-producing strains.
• Diagnostic Kit Development: Serves as a key component in the development of commercial diagnostic kits for enzyme activity measurement.

Basic Information

Product Name	p-Nitrophenyl β-D-Xylobioside
CAS No.	6819-07-4
Molecular Formula	C₁₇H₂₃NO₁₂
Molecular Weight	433.36 g/mol
Synonyms	4-Nitrophenyl β-D-xylobioside; p-Nitrophenyl β-D-xylobioside; 4-Nitrophenyl β-xylobioside; PNX; pNP-β-Xylobioside; 1,4-β-D-Xylobioside 4-nitrophenyl; 4-Nitrophenyl 4-O-β-D-xylopyranosyl-β-D-xylopyranoside
EINECS	Contact for details

Quality Control

Our p-Nitrophenyl β-D-Xylobioside is produced under strict quality management protocols. Each batch undergoes rigorous analytical testing, including HPLC for purity verification and identity confirmation by spectroscopic methods, to ensure it meets the high standards required for enzymatic research. A comprehensive Certificate of Analysis (COA) detailing purity, assay, and impurity profiles is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C. This product is hygroscopic (moisture-sensitive) and should be handled in a dry environment to prevent degradation. For long-term storage, consider desiccation.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 2.0%
Melting Point	Contact for details
Specific Rotation	Contact for details

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.