Description

D-Amethopterin Hydrate, 95 CAS NO 6745-93-3 is a high-purity, 95% grade of the methotrexate hydrate compound, a key folic acid antagonist. This product is essential for research and development in pharmaceutical chemistry, particularly in studying antimetabolite mechanisms and developing therapeutic agents. It is primarily needed by research institutions, pharmaceutical R&D laboratories, and fine chemical manufacturers focused on oncology and autoimmune disease research.

Application

• Pharmaceutical Reference Standard: Used as a certified reference material (CRM) for quality control and method validation in drug analysis.
• Biochemical Research: Serves as a critical tool in studying dihydrofolate reductase (DHFR) inhibition and folate metabolism pathways.
• Preclinical Studies: Employed in laboratory research for investigating the pharmacokinetics and pharmacodynamics of methotrexate analogs.
• Fine Chemical Synthesis: Acts as a key intermediate or starting material in the synthesis of specialized chemical derivatives for research purposes.
• Academic Research: Utilized in university and institutional labs for educational and investigative projects in medicinal chemistry.

Basic Information

Product Name	D-Amethopterin Hydrate, 95
CAS No.	6745-93-3
Molecular Formula	C₂₀H₂₂N₈O₅ • xH₂O
Molecular Weight	454.44 g/mol (anhydrous basis)
Synonyms	Methotrexate Impurity D; (2S)-2-[[4-[(2,4-Diaminopteridin-6-yl)methyl-methylamino]benzoyl]amino]pentanedioic acid hydrate; L-Glutamic acid, N-[4-[[(2,4-diamino-6-pteridinyl)methyl]methylamino]benzoyl]-, hydrate (1:?); Amethopterin Impurity D Hydrate; MTX Impurity D
EINECS	Contact for details

Quality Control

Our D-Amethopterin Hydrate is produced and tested under a strict quality management system. Each batch undergoes comprehensive analysis to confirm identity, purity, and consistency. Certificates of Analysis (COA) detailing specific results for assay, related substances, and residual solvents are provided and available upon request to support your quality and regulatory requirements.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This material is hygroscopic (moisture-sensitive) and must be kept in a dry environment. For long-term storage, consider desiccated conditions.

Specification

Item	Specification
Appearance	Yellow to orange crystalline powder
Identification (IR)	Conforms to reference spectrum
Assay (HPLC)	NLT 95.0%
Related Substances (HPLC)	Total impurities: NMT 5.0% Any individual impurity: NMT 1.0%
Water Content (KF)	Reported on COA
Residue on Ignition	NMT 0.5%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.