Description

1-Leucine-4-Nitroanilide is a specialized chromogenic substrate widely used in biochemical research and diagnostic applications. This compound is valued for its role as a key reagent in enzymatic assays, particularly for the detection and quantification of protease activity. It is an essential component for researchers and manufacturers in the pharmaceutical, biotechnology, and clinical diagnostics sectors who require reliable, high-purity substrates for their analytical workflows.

Application

• Protease Activity Assays: Primary substrate for colorimetric detection of leucine aminopeptidase and related proteolytic enzymes.
• Biochemical Research: Used in enzyme kinetics studies, inhibitor screening, and mechanistic investigations of peptidases.
• Diagnostic Kit Manufacturing: A critical raw material in the production of diagnostic test kits for liver function and other clinical markers.
• Pharmaceutical Development: Employed in drug discovery pipelines for target validation and high-throughput screening (HTS) assays.
• Quality Control in Biotechnology: Used to monitor enzyme purity and activity during the production of biopharmaceuticals.
• Academic Research: A standard reagent in university and institutional labs for teaching and advanced research in enzymology.

Basic Information

Product Name	1-Leucine-4-Nitroanilide
CAS No.	6664-98-8
Molecular Formula	C12H17N3O4
Molecular Weight	267.28 g/mol
Synonyms	L-Leucine-4-nitroanilide; L-Leu-pNA; Leu-pNA; 4-Nitroanilide of L-leucine; N-(4-Nitrophenyl)-L-leucinamide; L-Leucyl-4-nitroaniline; L-Leucine p-nitroanilide; Substrate for leucine aminopeptidase
EINECS	Contact for details

Quality Control

Our 1-Leucine-4-Nitroanilide is produced under strict quality management systems to ensure batch-to-batch consistency and high performance in sensitive applications. We provide comprehensive analytical documentation, including Certificates of Analysis (COA) with detailed HPLC purity data, identity confirmation (IR), and impurity profiles. Our quality commitment aligns with the standards expected for research-grade and diagnostic-grade biochemicals.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This product is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use to prevent degradation from atmospheric moisture.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Assay (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Melting Point	Contact for details
Heavy Metals	< 20 ppm
Solubility	Soluble in DMSO, DMF; slightly soluble in ethanol

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.