Description

3-Hydroxy-5-Cholestenoic Acid is a significant oxysterol and an intermediate metabolite in the bile acid biosynthesis pathway. This compound matters for researchers and manufacturers as a critical reference standard and a key biochemical precursor for studying cholesterol metabolism and related disorders. It is primarily needed by the pharmaceutical, biotechnology, and academic research sectors for analytical method development, metabolic pathway studies, and the synthesis of more complex steroidal compounds.

Application

• Biochemical Research: Used as a high-purity reference standard in LC-MS, GC-MS, and HPLC analyses for quantifying oxysterols in biological samples.
• Pharmaceutical Development: Serves as a key intermediate in the research and synthesis of novel therapeutic agents targeting cholesterol-related diseases.
• Metabolic Studies: Essential for investigating the alternative acidic pathway of bile acid biosynthesis and understanding associated metabolic disorders.
• Diagnostic Reagent Manufacturing: Employed in the production of calibrators and controls for clinical diagnostic kits measuring sterol metabolites.
• Academic Research: A vital tool for universities and research institutes studying liver function, atherosclerosis, and neurodegenerative diseases linked to cholesterol metabolism.

Basic Information

Product Name	3-Hydroxy-5-Cholestenoic Acid
CAS No.	6561-58-6
Molecular Formula	C27H44O3
Molecular Weight	416.64 g/mol
Synonyms	5-Cholestene-3β-ol-26-oic Acid; 3β-Hydroxy-5-cholestenoic Acid; (25R)-3β-Hydroxy-5-cholesten-26-oic Acid; 3-Hydroxycholest-5-en-26-oic Acid; 26-Carboxy-5-cholesten-3β-ol; 5-Cholestene-3β,26-diol-26-oic Acid; 3β-Hydroxy-5-cholesten-26-oate
EINECS	Contact for details

Quality Control

Our 3-Hydroxy-5-Cholestenoic Acid is produced under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation via spectroscopic methods, to ensure it meets the high standards required for research and development. Certificates of Analysis (COA) with detailed chromatographic data are available upon request to support your quality assurance protocols.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at controlled room temperature (15-25°C). This product is hygroscopic (moisture-sensitive) and light-sensitive; ensure the container is sealed tightly after each use to minimize exposure to air and humidity.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Identification (HPLC)	Retention time matches reference standard
Assay (HPLC)	≥ 95.0%
Related Substances (HPLC)	Total impurities ≤ 5.0%
Loss on Drying	≤ 2.0%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.