Description

2,3-Diphenyl-5-Methyltetrazolium Chloride is a vital tetrazolium salt widely used as a redox indicator and a key reagent in biochemical assays. Its primary value lies in its ability to form intensely colored formazan dyes upon reduction, making it indispensable for measuring cellular metabolic activity and dehydrogenase enzyme function. This compound is critically needed by research institutions, pharmaceutical developers, and quality control laboratories for applications in cytotoxicity testing, cell proliferation assays, and microbiological studies.

Application

• Cell Viability and Proliferation Assays: Used as the core substrate in MTT and related assays to quantify mitochondrial activity in live cells.
• Microbiological Testing: Employed to assess microbial growth, viability, and metabolic activity in environmental and clinical samples.
• Enzyme Activity Studies: Serves as an electron acceptor in dehydrogenase enzyme assays, including those for lactate dehydrogenase (LDH).
• Cytotoxicity Screening: A standard reagent for in vitro toxicology studies to evaluate the effects of chemicals, drugs, and nanomaterials on cell health.
• Biochemical Research: Utilized in various research protocols to study oxidative stress, apoptosis, and other cellular processes.
• Pharmaceutical Development: Applied in drug discovery for high-throughput screening of compound libraries for biological activity.

Basic Information

Product Name	2,3-Diphenyl-5-Methyltetrazolium Chloride
CAS No.	6275-01-0
Molecular Formula	C20H17ClN4
Molecular Weight	348.83 g/mol
Synonyms	MTT; MTT Tetrazolium; MTT Chloride; Thiazolyl Blue Tetrazolium Bromide (common misnomer); 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium Bromide (related compound name); Tetrazolium Blue Chloride; MTT Reagent; Cell Proliferation Kit Reagent
EINECS	Contact for details

Quality Control

Our 2,3-Diphenyl-5-Methyltetrazolium Chloride is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing to ensure high purity, consistent performance, and suitability for sensitive biochemical applications. A comprehensive Certificate of Analysis (COA) detailing purity, identity, and impurity profiles is provided with every shipment to support your research and quality assurance protocols.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 15-25°C. This material is hygroscopic (moisture-sensitive); ensure the container is kept tightly sealed in a low-humidity environment to maintain stability and performance.

Specification

Item	Specification
Appearance	Yellow to orange powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Loss on Drying	≤ 2.0%
Solubility	Soluble in water, ethanol, DMSO
Absorbance (in solution)	Conforms to typical profile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.