Description

Tiglyl-Coenzyme A is a crucial biochemical intermediate, specifically an acyl-CoA thioester derivative of tiglic acid. This compound plays a vital role in metabolic pathways, particularly in the catabolism of isoleucine and the biosynthesis of secondary metabolites. It is an essential research reagent for enzymologists and biochemists studying mitochondrial metabolism, fatty acid oxidation, and metabolic disorders. Key industries that utilize this high-purity biochemical include pharmaceutical research, diagnostic development, and academic life sciences.

Application

• Metabolic Pathway Research: Critical substrate for studying the degradation of the branched-chain amino acid isoleucine via the tiglyl-CoA pathway.
• Enzyme Kinetics & Assay Development: Used as a specific substrate for characterizing the activity of enzymes like 2-methylacyl-CoA dehydrogenase (ACADs) and other acyl-CoA dehydrogenases.
• Biochemical Standard: Serves as a high-purity reference standard in mass spectrometry (MS) and liquid chromatography (LC) for the quantification of acyl-CoA species in biological samples.
• Study of Metabolic Disorders: Research into inborn errors of metabolism, such as disorders of isoleucine catabolism (e.g., 2-methylbutyryl-CoA dehydrogenase deficiency).
• Mitochondrial Function Analysis: Investigation of mitochondrial β-oxidation defects and energy metabolism in cell and animal models.
• Biosynthesis Studies: Probe for understanding the biosynthesis of complex natural products derived from propionate units.

Basic Information

Product Name	Tiglyl-Coenzyme A
CAS No.	6247-62-7
Molecular Formula	C₂₆H₄₂N₇O₁₇P₃S
Molecular Weight	849.63 g/mol
Synonyms	Tiglyl-CoA; (E)-2-Methyl-2-butenoyl-CoA; Tiglyl-coenzyme A lithium salt; Tiglyl-S-coenzyme A; 2-Methylcrotonoyl-CoA; Coenzyme A, S-(2-methyl-2-butenoate); S-[2-Methyl-2-butenoate]coenzyme A; Tiglic acid CoA ester
EINECS	Contact for details

Quality Control

Our Tiglyl-Coenzyme A is manufactured under strict quality protocols to ensure batch-to-batch consistency and high biochemical purity suitable for sensitive research applications. Each lot undergoes comprehensive analytical testing, including HPLC for purity assessment and identity confirmation via mass spectrometry. A Certificate of Analysis (COA) detailing purity, identity, and other critical parameters is provided with every shipment to support your quality assurance and regulatory documentation needs.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive). For long-term storage, consider desiccant and under an inert atmosphere to maintain stability. Allow the vial to equilibrate to room temperature before opening to minimize moisture condensation.

Specification

Item	Specification
Appearance	White to off-white powder or lyophilized solid
Identification (HPLC/MS)	Conforms to reference standard
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 5.0 %
Biological Activity	Functional in standard enzyme assays

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.