Description

Benzoylarginine-p-Nitroanilide (CAS NO 6208-93-1) is a chromogenic peptide substrate widely used in enzymatic assays. This compound is critical for the precise measurement of trypsin-like protease activity, particularly in biochemical and pharmaceutical research. It is an essential reagent for researchers and quality control laboratories in the life sciences, diagnostic, and pharmaceutical development sectors.

Application

• Primary Substrate for Trypsin and Trypsin-like Proteases: Used in kinetic and endpoint assays to determine enzyme activity and concentration.
• Biochemical Research: Fundamental tool for studying protease kinetics, inhibition, and enzyme mechanism elucidation.
• Pharmaceutical Quality Control: Employed in the potency testing and standardization of therapeutic enzymes.
• Diagnostic Reagent Manufacturing: A key component in the formulation of diagnostic kits for pancreatic function and other clinical tests.
• Cell Biology and Physiology Studies: Used to investigate the role of specific proteases in cellular processes and disease pathways.

Basic Information

Product Name	Benzoylarginine-p-Nitroanilide
CAS No.	6208-93-1
Molecular Formula	C20H23N7O5
Molecular Weight	441.44 g/mol
Synonyms	BAPNA; Nα-Benzoyl-DL-arginine 4-nitroanilide hydrochloride; Benzoyl-Arg-pNA; Nα-Benzoyl-DL-arginine p-nitroanilide; Bz-Arg-pNA; Nα-Benzoyl-DL-arginine-4-nitroanilide; Benzoyl-L-arginine p-nitroanilide; Trypsin substrate BAPNA
EINECS	Contact for details

Quality Control

Our Benzoylarginine-p-Nitroanilide is produced under strict quality management protocols to ensure high purity and batch-to-batch consistency suitable for sensitive research applications. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and performance in standard assays. We adhere to relevant industry standards for biochemical reagents.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 15-25°C. This material is hygroscopic (moisture-sensitive) and light-sensitive; ensure containers are sealed tightly after each use to prevent degradation and absorption of moisture.

Specification

Item	Specification
Appearance	Yellow to light yellow crystalline powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content	≤ 1.0%
Melting Point	285 - 295 °C (dec.)
Absorbance (λ max)	310 - 320 nm
Solubility	Soluble in DMSO, DMF; slightly soluble in water
Performance (Enzymatic Assay)	Suitable for use as a trypsin substrate

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.