Description

Lysine 4-Nitroanilide is a specialized chromogenic substrate widely used in enzymatic assays and biochemical research. This compound is critical for the detection and quantification of protease activity, particularly for enzymes like plasmin and trypsin. It is an essential reagent for researchers and quality control laboratories in the pharmaceutical, diagnostic, and life science industries seeking reliable and sensitive assay components.

Application

• Protease Activity Assays: Primary substrate for the colorimetric determination of plasmin, trypsin, and related serine proteases.
• Diagnostic Kit Manufacturing: A key component in the formulation of diagnostic kits for clinical analysis of blood coagulation and fibrinolysis.
• Biochemical Research: Used in enzyme kinetics studies, inhibitor screening, and mechanistic studies of proteolytic enzymes.
• Pharmaceutical Quality Control: Employed in purity and potency testing of biopharmaceuticals where protease activity is a critical quality attribute.
• Food Science & Safety: Applied in research to analyze proteolytic enzyme activity in food products and processing.

Basic Information

Product Name	Lysine 4-Nitroanilide
CAS No.	6184-11-8
Molecular Formula	C12H18N4O4
Molecular Weight	282.30 g/mol
Synonyms	L-Lysine 4-nitroanilide; H-Lys-pNA; L-Lysine-p-nitroanilide; Nα-Benzoyl-L-lysine 4-nitroanilide; Bz-Lys-pNA; Plasmin Substrate; S-2251 (H-D-Val-Leu-Lys-pNA) related fragment; 4-Nitroanilide of L-lysine; N-α-Benzoyl-L-lysine 4-nitroanilide hydrochloride (salt form)
EINECS	Contact for details

Quality Control

Our Lysine 4-Nitroanilide is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity and identity confirmation (IR, NMR), to ensure it meets the high standards required for research and diagnostic applications. Certificates of Analysis (COA) with lot-specific data are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 15-25°C. This product is hygroscopic (moisture-sensitive) and should be handled in a dry environment to prevent degradation.

Specification

Item	Specification
Appearance	Yellow to light brown crystalline powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Melting Point	Contact for details
Solubility	Soluble in DMSO, DMF; slightly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.