Description

Uracil-5-3H is a tritium-labeled derivative of the essential pyrimidine nucleobase uracil, specifically radiolabeled at the 5-position. This compound is a critical tool for researchers requiring high-sensitivity detection and precise metabolic pathway tracing in biochemical and pharmacological studies. It is primarily utilized by scientists in pharmaceutical R&D, academic biochemistry, and molecular biology for applications such as nucleic acid metabolism research and drug mechanism of action studies.

Application

• Biochemical Tracer Studies: Used to investigate the metabolism, synthesis, and catabolism of pyrimidine nucleotides and nucleic acids in vitro and in vivo.
• Drug Discovery & Development: Employed in radioligand binding assays and ADME (Absorption, Distribution, Metabolism, Excretion) studies to track the incorporation and fate of nucleoside analogs.
• Molecular Biology Research: Serves as a precursor for studying RNA synthesis, repair mechanisms, and the function of uracil-DNA glycosylase.
• Oncology Research: Applied in studies targeting nucleotide metabolism in cancer cells, aiding in the development of chemotherapeutic agents.
• Enzymology: Acts as a substrate for enzymes like dihydropyrimidine dehydrogenase (DPD) to study enzyme kinetics and inhibition.
• Autoradiography & Imaging: Provides a radioactive tag for precise localization and quantification in tissue sections or chromatographic analyses.

Basic Information

Product Name	Uracil-5-3H
CAS No.	6165-71-5
Molecular Formula	C₄H₃D₃N₂O₂
Molecular Weight	114.10 g/mol (unlabeled); Specific activity provided on COA.
Synonyms	5-Tritiouracil; [5-³H]Uracil; 2,4-Dihydroxypyrimidine-5-t; Uracil (5-³H); 5-³H-Uracil; Uracil-5-t; Uracil tritiated at C-5; NSC 2100 (³H-labeled)
EINECS	228-220-3 (for unlabeled uracil)

Quality Control

Every batch of Uracil-5-3H is manufactured and analyzed under strict quality systems. Radiochemical purity and specific activity are determined via validated methods such as HPLC with radiometric detection. A comprehensive Certificate of Analysis (COA) is supplied with each shipment, detailing specific activity, purity, solvent composition, and analytical results. Our quality commitment ensures the material meets the stringent requirements for sensitive life science research.

Storage

Preserve in a tightly closed container, protected from light. Store at or below -20°C in the original solvent as specified on the label and COA. For long-term storage, keep in a dedicated freezer away from strong radiation sources. Users must follow all local and international regulations for the safe handling and disposal of radioactive materials.

Specification

Item	Specification
Appearance	Clear solution (color may vary)
Identification (HPLC)	Conforms to reference standard
Specific Activity	Customizable, typically 10-30 Ci/mmol
Radiochemical Purity (HPLC)	≥ 97%
Chemical Purity (HPLC)	≥ 98%
Solvent	Ethanol:Water mixture (specified on COA)
Concentration	1 mCi/mL (as specified on COA)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.