Description

4-Methylumbelliferyl 2,3,4,6-Tetra-o-Acetyl-β-D-Galactopyranoside is a specialized fluorogenic substrate used for the detection and quantification of β-galactosidase enzyme activity. This compound matters because it provides a highly sensitive and specific means for enzymatic assays, releasing the fluorescent 4-methylumbelliferone upon cleavage. It is essential for researchers and quality control laboratories in the biotechnology, pharmaceutical, and diagnostic industries, particularly for applications in reporter gene assays, cell biology studies, and enzyme-linked immunosorbent assays (ELISA).

Application

• Enzymatic Assays: Primary substrate for the fluorometric detection of β-galactosidase activity in research and diagnostic kits.
• Reporter Gene Systems: Used in molecular biology to monitor gene expression and promoter activity in cell-based assays.
• Diagnostic Development: A key component in the development of clinical diagnostic tests for enzymatic deficiencies, such as lysosomal storage disorders.
• Microbiology & Cell Biology: Employed in studies of bacterial gene regulation (e.g., lacZ gene) and for staining cells expressing β-galactosidase.
• High-Throughput Screening (HTS): Utilized in drug discovery platforms to identify modulators of β-galactosidase activity or related pathways.
• Biochemical Research: Serves as a tool for kinetic studies, enzyme characterization, and inhibition profiling.
• Quality Control: Applied in the biomanufacturing sector to test for the presence of microbial contamination via enzymatic markers.

Basic Information

Product Name	4-Methylumbelliferyl 2,3,4,6-Tetra-o-Acetyl-β-D-Galactopyranoside
CAS No.	6160-79-8
Molecular Formula	C26H28O14
Molecular Weight	564.49 g/mol
Synonyms	4-MU-β-D-Galactopyranoside tetraacetate; 4-Methylumbelliferyl β-D-galactopyranoside tetraacetate; 4-Methylumbelliferyl-2,3,4,6-tetra-O-acetyl-β-D-galactopyranoside; 4-MUGal tetraacetate; 4-MU-β-Gal tetraacetate; β-Galactosidase substrate, fluorogenic; 4-Methylumbelliferyl tetraacetyl-β-D-galactoside
EINECS	Contact for details

Quality Control

Our 4-Methylumbelliferyl 2,3,4,6-Tetra-o-Acetyl-β-D-Galactopyranoside is manufactured under strict quality systems to ensure batch-to-batch consistency and high purity suitable for sensitive biochemical applications. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and performance in standard enzymatic assays. We adhere to relevant quality guidelines to support your research and development needs.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C in a dry environment. This product is hygroscopic (moisture-sensitive) and light-sensitive; prolonged exposure to moisture, heat, or light should be avoided to maintain stability and performance.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Melting Point	Contact for details
Water Content (KF)	≤ 1.0%
Specific Rotation	Contact for details
Fluorogenic Activity	Conforms to reference standard

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.