Description

4-Methylumbelliferyl β-D-Galactoside is a fluorogenic substrate specifically designed for the detection and quantification of β-galactosidase enzyme activity. This compound is critical for research and diagnostic applications where high sensitivity and precise enzymatic measurement are required. It is primarily utilized by professionals in life science research, clinical diagnostics, and biotechnology for applications such as gene expression studies, cell-based reporter assays, and enzyme-linked immunosorbent assays (ELISAs).

Application

• Enzyme Activity Assays: A standard fluorogenic substrate for the sensitive detection and kinetic analysis of β-galactosidase (β-Gal) activity in vitro.
• Reporter Gene Assays: Widely used in molecular biology as a reporter substrate in systems like the lacZ gene, enabling the visualization and quantification of gene expression in cells.
• Microbiology & Diagnostics: Employed in diagnostic kits and microbiological tests for the identification of bacterial species (e.g., E. coli) based on β-galactosidase production.
• High-Throughput Screening (HTS): Ideal for cell-based and biochemical HTS campaigns in drug discovery to identify modulators of gene expression or enzyme function.
• Histochemistry & Cell Biology: Used in staining protocols to localize β-galactosidase activity in tissues or fixed cells, often in senescence-associated β-galactosidase (SA-β-gal) assays.
• Quality Control in Biotechnology: Applied in the quality control of recombinant proteins and enzyme preparations to verify specific activity and purity.

Basic Information

Product Name	4-Methylumbelliferyl β-D-Galactoside
CAS No.	6160-78-7
Molecular Formula	C16H18O9
Molecular Weight	354.31 g/mol
Synonyms	4-MU-β-D-Galactoside; 4-Methylumbelliferyl-β-D-galactopyranoside; β-Galactosidase Substrate; 4-MUG; 4-Methylumbelliferyl β-D-galactopyranoside; 4-MU-Gal; 7-Hydroxy-4-methylcoumarin β-D-galactopyranoside
EINECS	228-200-0

Quality Control

Our 4-Methylumbelliferyl β-D-Galactoside is manufactured under strict quality management systems to ensure batch-to-batch consistency and high performance in sensitive assays. Each lot is subjected to rigorous analytical testing, including HPLC for purity and identity confirmation via spectroscopic methods. A comprehensive Certificate of Analysis (COA) detailing purity, assay results, and impurity profiles is provided with every shipment to support your research and compliance needs.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C in a dry environment. The product is hygroscopic (moisture-sensitive) and light-sensitive (store away from light). For long-term storage, desiccate at -20°C.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 2.0%
Fluorogenic Activity	Suitable for enzyme assays
Solubility	Soluble in DMSO, DMF; slightly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.