Description

L-Arginine p-Nitroanilide Dihydrobromide is a specialized chromogenic substrate widely used in enzymatic assays and biochemical research. This compound is critical for the sensitive and specific detection of trypsin-like proteases, including thrombin and plasmin, due to its release of p-nitroaniline upon cleavage. It is an essential reagent for researchers and quality control laboratories in the pharmaceutical, diagnostic, and life science sectors requiring precise enzyme activity measurement. The product is supplied as a high-purity material to ensure reliable and reproducible experimental results.

Application

• Protease Activity Assays: Primary substrate for the kinetic and endpoint determination of trypsin, thrombin, and other serine proteases.
• Diagnostic Kit Manufacturing: A key component in the production of in-vitro diagnostic (IVD) kits for coagulation disorders and other clinical assays.
• Biochemical Research: Used in enzyme characterization, inhibitor screening, and mechanistic studies in academic and industrial R&D laboratories.
• Pharmaceutical Quality Control: Employed in potency testing and stability studies of biopharmaceuticals that involve protease activity.
• High-Throughput Screening (HTS): Suitable for automated screening platforms in drug discovery to identify novel protease inhibitors or activators.

Basic Information

Product Name	L-Arginine p-Nitroanilide Dihydrobromide
CAS No.	6154-84-3
Molecular Formula	C12H20Br2N6O3
Molecular Weight	464.14 g/mol
Synonyms	BAPNA; Nα-Benzoyl-DL-arginine p-nitroanilide hydrochloride; Benzoyl-Arg-pNA; Bz-Arg-pNA; L-BAPA; Nα-Benzoyl-L-arginine 4-nitroanilide hydrochloride; Nα-Benzoyl-L-arginine p-nitroanilide hydrochloride; Bz-L-Arg-pNA
EINECS	Contact for details

Quality Control

Our L-Arginine p-Nitroanilide Dihydrobromide is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity, identity confirmation by IR and NMR spectroscopy, and residual solvent analysis. We provide full traceability and Certificates of Analysis (COA) are supplied with every shipment, detailing all test results against established in-house specifications. Our commitment ensures material suitability for sensitive research and diagnostic applications.

Storage

Preserve in a tightly closed container, protected from light. Store at controlled room temperature (15-25°C) in a dry environment. This material is hygroscopic (moisture-sensitive); ensure the container is sealed after each use to prevent absorption of atmospheric moisture, which may affect performance and stability.

Specification

Item	Specification
Appearance	Yellow to orange crystalline powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 2.0%
Heavy Metals	≤ 20 ppm
Solubility	Clear, yellow solution in DMSO

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.