Description

Acetylthiocholine Chloride is a high-purity biochemical reagent primarily used as a substrate for the enzyme acetylcholinesterase. This compound is critical for research and diagnostic applications where precise enzymatic activity measurement is required. It is essential for scientists and manufacturers in the fields of neurobiology, toxicology, and clinical diagnostics. Our product, CAS NO 6050-81-3, is supplied with rigorous quality control to ensure reliable and reproducible results in sensitive assays.

Application

• Acetylcholinesterase (AChE) Activity Assays: The primary substrate for colorimetric (Ellman's method) and electrochemical assays to measure AChE enzyme activity in research and diagnostic kits.
• Neuropharmacology & Toxicology Research: Used in studies investigating the effects of neuroactive compounds, pesticides (organophosphates, carbamates), and nerve agents on cholinergic systems.
• Clinical Diagnostics: Employed in vitro diagnostic (IVD) test systems for the detection of cholinesterase levels in blood, which is a biomarker for certain liver conditions and pesticide exposure.
• Drug Discovery & Screening: A key reagent in high-throughput screening (HTS) platforms to identify potential inhibitors or activators of acetylcholinesterase for therapeutic development.
• Environmental Monitoring: Used in biosensor development and test kits for detecting anticholinesterase agents in water, soil, and food samples.
• Biochemical Research: Serves as a standard and reference material in kinetic studies and for the calibration of analytical instruments measuring thiol production.

Basic Information

Product Name	Acetylthiocholine Chloride
CAS No.	6050-81-3
Molecular Formula	C7H16ClNOS
Molecular Weight	197.73 g/mol
Synonyms	Acetylthiocholine chloride; (2-Acetylthioethyl)trimethylammonium chloride; ATCh; ATChCl; ATC; Acetylthiocholine iodide analog (chloride salt); 2-Acetamidoethylthiocholine chloride; Thioacetylcholine chloride
EINECS	227-984-4

Quality Control

Our Acetylthiocholine Chloride is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity and identity confirmation via IR and NMR spectroscopy, to ensure it meets the stringent requirements for research and diagnostic applications. A detailed Certificate of Analysis (COA) is provided with every shipment, documenting purity, assay, and impurity profiles.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C. This product is hygroscopic (moisture-sensitive) and should be handled in a dry environment to prevent degradation. For long-term stability, consider storage under desiccant or inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Identification (NMR)	Conforms to structure
Assay (HPLC)	≥ 98.0%
Water Content (KF)	≤ 2.0%
Residue on Ignition	≤ 0.1%
Heavy Metals (as Pb)	≤ 20 ppm
Related Substances (HPLC)	Total impurities ≤ 2.0%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.