Description

9-Biotinlamino-4,7-Dioxanonanoic Acid n-Hydroxysuccinimidyl Ester is a high-purity, heterobifunctional crosslinking reagent designed for the site-specific biotinylation of proteins, peptides, and other biomolecules. This compound is critical for researchers and manufacturers requiring stable, covalent attachment of biotin tags to primary amines, enabling downstream detection, purification, or immobilization via the strong streptavidin-biotin interaction. It is an essential tool for professionals in bioconjugation, diagnostic assay development, proteomics research, and pharmaceutical R&D.

Application

• Bioconjugation and Labeling: Covalent attachment of biotin to antibodies, enzymes, and other proteins for immunoassays (ELISA, Western Blot) and diagnostic kits.
• Affinity Purification Systems: Preparation of biotinylated ligands for capture on streptavidin or avidin resins.
• Proteomics and Protein Interaction Studies: Tagging proteins for pull-down assays, mass spectrometry analysis, and protein microarray fabrication.
• Drug Delivery and Targeting: Conjugation of biotin to drug molecules or nanoparticles for targeted delivery systems utilizing biotin receptors.
• Cell Surface Engineering: Labeling of cell surface amines for fluorescence-activated cell sorting (FACS) or microscopy studies.
• Biosensor Development: Immobilization of biorecognition elements onto biotin-functionalized sensor surfaces.
• Nucleic Acid Probes: Biotinylation of amine-modified oligonucleotides for in situ hybridization and genomic detection.

Basic Information

Product Name	9-Biotinlamino-4,7-Dioxanonanoic Acid n-Hydroxysuccinimidyl Ester
CAS No.	596820-83-6
Molecular Formula	C23H37N5O8S
Molecular Weight	543.63 g/mol
Synonyms	Biotin-PEG3-NHS Ester; Biotinamidocaproyl-PEG3-NHS; Biotin-PEG3-NHS; Biotin-(PEG)3-NHS Ester; NHS-PEG3-Biotin; Biotinamidohexanoyl-PEG3-NHS Ester; 2,5-Dioxopyrrolidin-1-yl 3-(2-(2-(2-(5-((3aS,4S,6aR)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanamido)ethoxy)ethoxy)ethoxy)propanoate; Biotinylated PEG NHS Ester
EINECS	Contact for details

Quality Control

Our 9-Biotinlamino-4,7-Dioxanonanoic Acid n-Hydroxysuccinimidyl Ester is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity, NMR for structural confirmation, and mass spectrometry for identity verification, to ensure it meets the high standards required for sensitive life science applications. Certificates of Analysis (COA) with detailed chromatographic data are provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This product is hygroscopic (moisture-sensitive) and should be allowed to equilibrate to room temperature in a dry environment before opening to prevent condensation and hydrolysis.

Specification

Item	Specification
Appearance	White to off-white powder or solid
Identification (HPLC/MS)	Conforms to reference standard
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF, acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.