Description

Mca-Gly-Ser-Pro-Ala-Phe-Leu-Ala-Lys(Dnp)-D-Arg-Nh2 is a specialized, high-purity synthetic peptide sequence containing a 7-methoxycoumarin-4-acetyl (Mca) fluorophore and a 2,4-dinitrophenyl (Dnp) quencher group. This compound matters as a critical fluorogenic substrate for the study and detection of specific protease enzyme activity, particularly in biochemical research and diagnostic assay development. It is primarily needed by pharmaceutical R&D laboratories, diagnostic kit manufacturers, and academic research institutions focused on enzymology, high-throughput screening, and biomarker discovery.

Application

• Protease Activity Assays: Used as a selective fluorogenic substrate for monitoring the kinetics and inhibition of specific proteolytic enzymes in vitro.
• High-Throughput Screening (HTS): Employed in drug discovery platforms to identify and characterize novel protease inhibitors for therapeutic targets.
• Diagnostic Development: Serves as a key component in the development of sensitive fluorometric diagnostic tests for detecting abnormal protease levels associated with diseases.
• Biochemical Research: Utilized in academic and industrial labs to study enzyme mechanism, specificity, and regulation within complex biological pathways.
• Quality Control Reagent: Acts as a reference standard or critical reagent in the quality control of enzyme preparations and related biopharmaceutical products.

Basic Information

Item	Details
Product Name	Mca-Gly-Ser-Pro-Ala-Phe-Leu-Ala-Lys(Dnp)-D-Arg-Nh2
CAS No.	592544-81-5
Molecular Formula	C58H78N16O19
Molecular Weight	1287.33 g/mol
Synonyms	7-Methoxycoumarin-4-acetyl-Gly-Ser-Pro-Ala-Phe-Leu-Ala-Lys(2,4-DNP)-D-Arg-amide; Mca-GSPAFLAK(Dnp)-D-R-NH2; Fluorogenic Peptide Substrate Mca-GSPAFLAK(Dnp)-D-Arg-NH2; CAS 592544-81-5; Protease Substrate V; Thrombin Fluorogenic Substrate; (7-Methoxycoumarin-4-yl)acetyl-Gly-Ser-Pro-Ala-Phe-Leu-Ala-Lys(2,4-dinitrophenyl)-D-argininamide
EINECS	Contact for details

Quality Control

Our Mca-Gly-Ser-Pro-Ala-Phe-Leu-Ala-Lys(Dnp)-D-Arg-Nh2 is manufactured under strict quality management systems to ensure batch-to-batch consistency and reliability for sensitive research applications. Each lot undergoes comprehensive analytical testing, including HPLC for purity verification and mass spectrometry for identity confirmation. Certificates of Analysis (COA) detailing all specifications and test results are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a freezer. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake. For long-term storage, keep desiccated.

Specification

Item	Specification
Appearance	Yellow to orange lyophilized powder
Identification (HPLC/MS)	Conforms to reference standard
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 5.0%
Peptide Content	≥ 70%
Solubility	Soluble in DMSO, DMF; slightly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.