Description

5'-O-Levulinoylthymidine 3'-CE Phosphoramidite is a high-purity, protected phosphoramidite monomer essential for the solid-phase synthesis of oligonucleotides. Its primary value lies in enabling the precise and automated construction of DNA sequences, particularly those requiring specialized modifications or high coupling efficiency. This critical building block is indispensable for researchers and manufacturers in the fields of therapeutic oligonucleotide development, molecular diagnostics, and genetic research.

Application

• Solid-Phase Oligonucleotide Synthesis: As a key monomer for automated DNA/RNA synthesizers.
• Therapeutic Oligonucleotide Manufacturing: Production of antisense oligonucleotides, siRNA, and aptamers for pharmaceutical applications.
• Probe and Primer Synthesis: Manufacture of labeled or modified probes for PCR, sequencing, and hybridization assays.
• Research-Grade Oligo Production: Synthesis of custom DNA sequences for academic and industrial R&D.
• Synthesis of Modified Nucleotides: Incorporation into oligonucleotides for studying structure-activity relationships or enhancing stability.

Basic Information

Product Name	5'-O-Levulinoylthymidine 3'-CE Phosphoramidite
CAS No.	581076-14-4
Molecular Formula	C33H43N4O8P
Molecular Weight	654.69 g/mol
Synonyms	5'-O-(4-Oxo-pentanoyl)thymidine 3'-(2-Cyanoethyl N,N-Diisopropylphosphoramidite); dT-LEV-CE Phosphoramidite; Thymidine, 5'-O-(4-oxopentanoyl)-, 3'-(2-cyanoethyl N,N-diisopropylphosphoramidite); Lev-dT-CE Phosphoramidite; 5'-Levulinyl-thymidine 3'-CE Phosphoramidite; 5'-O-Levulinyl-2'-deoxythymidine 3'-(2-cyanoethyl N,N-diisopropylphosphoramidite)
EINECS	Contact for details

Quality Control

Every batch of 5'-O-Levulinoylthymidine 3'-CE Phosphoramidite is manufactured and tested under strict quality systems to ensure it meets the exacting standards required for oligonucleotide synthesis. Our rigorous quality control includes comprehensive analytical testing by HPLC, NMR, and MS to verify identity, purity, and performance. Certificates of Analysis (COA) with full traceability are provided, supporting applications in GMP-compliant and research environments.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere (argon or nitrogen) in a dry, moisture-free environment. This product is hygroscopic and moisture-sensitive; allow the sealed vial to equilibrate to room temperature before opening to prevent condensation.

Specification

Item	Specification
Appearance	White to off-white solid or foam
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 0.5%
Solubility	Clear, colorless solution in anhydrous acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.