Description

Spdp-Peg4-Acid is a heterobifunctional crosslinker featuring an amine-reactive NHS ester and a thiol-reactive pyridyldithiol group, connected by a flexible tetraethylene glycol (PEG4) spacer. This compound is critical for creating stable, cleavable conjugates between proteins, peptides, or other biomolecules, enabling advanced research and therapeutic development. It is primarily utilized by scientists and manufacturers in bioconjugation, antibody-drug conjugate (ADC) development, and proteomics research.

Application

• Antibody-Drug Conjugate (ADC) Linker: A key reagent for constructing cleavable, PEGylated linkers that connect cytotoxic payloads to monoclonal antibodies.
• Protein-Protein/Peptide Conjugation: Enables the site-specific crosslinking of different biomolecules for creating bispecific constructs or protein complexes.
• Surface Functionalization: Used to modify nanoparticles, biosensors, or chromatographic resins with thiol-reactive groups for subsequent biomolecule immobilization.
• Bioconjugation Tool in Research: Facilitates the study of protein interactions, cellular uptake, and drug delivery mechanisms through reversible disulfide bond formation.
• Diagnostic Reagent Synthesis: Employed in the development of enzyme-linked or fluorescent immunoassays that require specific, cleavable linkages.

Basic Information

Product Name	Spdp-Peg4-Acid
CAS No.	581065-97-6
Molecular Formula	C₂₇H₄₀N₄O₁₂S₂
Molecular Weight	676.75 g/mol
Synonyms	SPDP-PEG4-acid; NHS-PEG4-SPDP; Succinimidyl-[(N-maleimidopropionamido)-tetraethyleneglycol] ester; 3-(2-Pyridyldithio)propionyl-PEG4-NHS ester; Maleimide-PEG4-SPDP; SMPT-PEG4-NHS; N-Succinimidyl 3-((2-(2-(2-(2-(3-(2-pyridyldithio)propanamido)ethoxy)ethoxy)ethoxy)ethoxy)propanamido)propanoate
EINECS	Contact for details

Quality Control

Our Spdp-Peg4-Acid is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity, NMR for structural confirmation, and mass spectrometry, to ensure it meets the high standards required for sensitive bioconjugation applications. A comprehensive Certificate of Analysis (COA) detailing purity, identity, and functional performance is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and hydrolysis. For long-term stability, store under inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white solid or oil
Identification (HPLC/MS)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%
Functional Activity	Conforms (by conjugation test)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.