Description

Mutanolysin is a highly specific bacteriolytic enzyme, also known as M1 muramidase, which selectively hydrolyzes the peptidoglycan cell walls of certain Gram-positive bacteria. This targeted action makes it an invaluable tool for research and industrial applications requiring the controlled lysis of bacterial cells. It is essential for laboratories and manufacturers in the biotechnology, pharmaceutical, and life science sectors. The product is supplied under the identifier CAS No. 554662-20-3.

Application

• Bacterial Protoplast Preparation: Essential for generating protoplasts from sensitive bacterial strains for genetic transformation and fusion studies.
• Cell Wall Analysis: Used in biochemical research to degrade peptidoglycan for structural and compositional analysis of bacterial cell walls.
• Enzyme Activity Assays: Serves as a critical reagent in assays designed to measure the activity of other cell wall-degrading enzymes or inhibitors.
• Protein Extraction: Facilitates the gentle release of intracellular proteins, enzymes, and other biomolecules from bacterial cells without damaging sensitive components.
• Diagnostic Kit Manufacturing: Incorporated into diagnostic kits and reagents for bacterial identification and susceptibility testing.
• Pharmaceutical Research: Employed in drug discovery programs targeting bacterial cell wall synthesis and integrity.

Basic Information

Product Name	Mutanolysin
CAS No.	554662-20-3
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	M1 Muramidase; N-Acetylmuramide Glycanhydrolase; Peptidoglycan N-acetylmuramoylhydrolase; Listeria ivanovii Bacteriophage Ply118 Lysin; Cellosyl; Lysozyme (from Streptomyces globisporus); Endo-β-N-acetylmuramidase
EINECS	Contact for details

Quality Control

Our Mutanolysin is produced and tested under stringent quality management systems to ensure batch-to-batch consistency and performance. Each lot undergoes comprehensive analysis to confirm enzymatic activity, purity, and the absence of contaminating proteases. A Certificate of Analysis (COA) detailing specific test results is provided with every shipment to support your quality assurance protocols.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C. This product is hygroscopic (moisture-sensitive) and should be handled in a dry environment to maintain stability and activity. For long-term storage, consider desiccated conditions.

Specification

Item	Specification
Appearance	White to off-white lyophilized powder
Identification (IR)	Conforms to reference standard
Assay (Enzymatic Activity)	≥ 5,000 units/mg protein
Protein Content (Biuret)	≥ 90%
Protease Activity	Not detected
Solubility	Clear, colorless solution in buffer
pH (1% solution)	6.0 - 7.5
Endotoxin (LAL)	< 1.0 EU/μg

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.