Description

H-Pro-Arg-Amc . 2 Hcl is a fluorogenic peptide substrate designed for the sensitive detection and measurement of protease activity. This compound matters for researchers and industrial scientists requiring precise enzymatic analysis, as it releases a fluorescent signal upon cleavage. It is primarily needed by professionals in pharmaceutical R&D, biochemical research, and diagnostic assay development for studying enzymes like thrombin, trypsin, and related serine proteases.

Application

• Protease Activity Assays: Fluorogenic substrate for continuous, real-time monitoring of enzyme kinetics for proteases such as thrombin, trypsin, and plasmin.
• Pharmaceutical Research & High-Throughput Screening (HTS): Used in drug discovery to identify and characterize potential protease inhibitors.
• Biochemical Research: Tool for studying protease specificity, enzyme mechanism, and pathway analysis in vitro.
• Diagnostic Development: Key component in developing sensitive clinical assays for detecting protease levels relevant to coagulation disorders or other diseases.
• Quality Control in Biologics: Monitoring protease activity during the production and purification of therapeutic proteins.

Basic Information

Product Name	H-Pro-Arg-Amc . 2 Hcl
CAS No.	546103-85-9
Molecular Formula	C22H33Cl2N7O4
Molecular Weight	546.45 g/mol
Synonyms	H-Pro-Arg-AMC dihydrochloride; Pro-Arg-7-amido-4-methylcoumarin dihydrochloride; Boc-Pro-Arg-AMC . 2HCl (deprotected form); Fluorogenic Thrombin Substrate I; Boc-P-R-AMC . 2HCl (TFA salt alternative); L-Prolyl-L-arginyl-7-amido-4-methylcoumarin dihydrochloride; Thrombin substrate, fluorogenic; Z-P-R-AMC . 2HCl (related derivative)
EINECS	Contact for details

Quality Control

Our H-Pro-Arg-Amc . 2 Hcl is produced under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation, to ensure it meets the high standards required for research and diagnostic applications. Certificates of Analysis (COA) with detailed specifications are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive) and light-sensitive (store away from light). Allow the vial to warm to room temperature in a desiccator before opening to minimize moisture absorption.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms to reference standard
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 5.0%
Solubility	Soluble in DMSO, Methanol, Water
Functional Activity	Conforms (Tested in standard thrombin assay)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.