Description

5-Bromo-4-Chloro-3-Indoxyl-β-D-Ribofuranoside is a specialized chromogenic substrate used in enzymatic assays and molecular biology research. This compound is critical for the sensitive detection and quantification of β-galactosidase activity, serving as a key reagent in reporter gene systems. It is primarily utilized by researchers and manufacturers in the pharmaceutical, biotechnology, and diagnostic industries for applications ranging from high-throughput screening to histological staining.

Application

• β-Galactosidase Reporter Gene Assays: A primary chromogenic substrate for detecting and quantifying β-galactosidase (lacZ) expression in molecular biology and genetic engineering.
• Histology and Cell Biology: Used in X-gal staining for visualizing gene expression patterns in tissues, embryos, and cultured cells (e.g., in situ hybridization).
• High-Throughput Screening (HTS): Enables the identification of enzyme inhibitors or activators in drug discovery pipelines.
• Microbial Detection: Employed in diagnostic media and kits for the identification of bacterial colonies expressing β-galactosidase, such as E. coli.
• Enzyme Kinetics Studies: Serves as a specific substrate for studying the kinetics and mechanism of β-galactosidase enzymes.
• Biochemical Research Reagent: A key component in buffer systems and kits for various biochemical analyses requiring precise enzyme activity measurement.

Basic Information

Product Name	5-Bromo-4-Chloro-3-Indoxyl-β-D-Ribofuranoside
CAS No.	518033-33-5
Molecular Formula	C13H13BrClNO6
Molecular Weight	394.60 g/mol
Synonyms	5-Bromo-4-chloro-3-indolyl β-D-ribofuranoside; BCIR; X-Riboside; 5-Bromo-4-chloro-1H-indol-3-yl β-D-ribofuranoside; 3-Indolyl β-D-ribofuranoside, 5-bromo-4-chloro-; β-D-Ribofuranoside, 5-bromo-4-chloro-3-indolyl; Chromogenic substrate for β-galactosidase
EINECS	Contact for details

Quality Control

Our 5-Bromo-4-Chloro-3-Indoxyl-β-D-Ribofuranoside is manufactured under strict quality systems to ensure batch-to-batch consistency and high purity suitable for sensitive research applications. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and performance in standard enzymatic assays. We adhere to ISO 9001 quality management principles, and our production environment is designed to meet the exacting standards of the life science research community.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 15-25°C. This product is hygroscopic (moisture-sensitive) and light-sensitive; therefore, containers must be kept sealed in a desiccated environment and opened under controlled conditions to minimize exposure to atmospheric moisture and light.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Melting Point	Contact for details
Solubility	Soluble in DMSO, DMF; slightly soluble in water
Heavy Metals	< 10 ppm
Residual Solvents (GC)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.