Description

Ddao Galactoside [9H-(1,3-Dichloro-9, 9-Dimethylacridin-2-One-7-Yl) β-D-Galactopyranoside] is a specialized fluorogenic substrate designed for high-sensitivity enzymatic detection. This compound matters for its critical role as a chromogenic and fluorogenic probe for β-galactosidase activity, enabling precise biochemical and cellular assays. It is primarily needed by researchers and manufacturers in the life sciences, pharmaceutical development, and diagnostic kit production for applications in reporter gene assays, cell staining, and enzyme activity monitoring.

Application

• Reporter Gene Assays: Used as a sensitive substrate for detecting β-galactosidase reporter gene expression in molecular biology and genetic engineering.
• Enzyme Activity Screening: Essential for high-throughput screening (HTS) of enzyme inhibitors or activators in drug discovery pipelines.
• Cell and Tissue Staining: Employed in histochemistry and cytochemistry for visualizing β-galactosidase activity in fixed cells and tissue sections.
• Diagnostic Development: Serves as a key component in the formulation of diagnostic kits for bacterial detection (e.g., E. coli) and other enzymatic biomarkers.
• Biochemical Research: Utilized in vitro to study enzyme kinetics, mechanism, and purification processes of β-galactosidase.
• Senescence Detection: Applied in assays to detect senescence-associated β-galactosidase (SA-β-gal) activity in aging and cancer research.

Basic Information

Product Name	Ddao Galactoside [9H-(1,3-Dichloro-9, 9-Dimethylacridin-2-One-7-Yl) β-D-Galactopyranoside]
CAS No.	503178-95-8
Molecular Formula	C24H23Cl2NO7
Molecular Weight	508.35 g/mol
Synonyms	DDAO Galactoside; 9H-(1,3-Dichloro-9,9-dimethylacridin-2-one-7-yl) β-D-galactopyranoside; DDAO-β-D-Galactopyranoside; 7-Hydroxy-9H-(1,3-dichloro-9,9-dimethylacridin-2-one) β-D-galactopyranoside; β-Galactosidase Substrate DDAO; Fluorogenic β-Gal Substrate; DDAO-β-Gal; (2S,3R,4S,5R,6R)-2-[(7-hydroxy-1,3-dichloro-9,9-dimethylacridin-2-yl)oxy]-6-(hydroxymethyl)oxane-3,4,5-triol
EINECS	Contact for details

Quality Control

Our Ddao Galactoside is produced under strict quality management systems to ensure batch-to-batch consistency and high purity for research and diagnostic applications. Each lot undergoes comprehensive analytical testing, including HPLC for purity assessment and spectroscopic confirmation (NMR, MS) of identity. Certificates of Analysis (COA) detailing all specifications are available upon request to support your quality assurance protocols.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the vial to equilibrate to room temperature before opening to minimize moisture absorption.

Specification

Item	Specification
Appearance	Yellow to orange solid
Identification (HPLC)	Conforms
Identification (MS)	Conforms
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 2.0%
Solubility	Soluble in DMSO, DMF; sparingly soluble in water
Extinction Coefficient	Contact for details
Fluorescence Properties	Contact for details

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.