Description

Neu5Ac Alpha(2-3)Gal Beta(1-4)Glcnac-β-Pnp is a high-purity, synthetic oligosaccharide derivative featuring a para-nitrophenyl (PNP) aglycon, designed for advanced biochemical research and diagnostic development. This compound serves as a critical chromogenic substrate for the specific detection and kinetic analysis of glycosidase and glycosyltransferase enzymes, particularly those involved in sialic acid metabolism. It is an essential tool for researchers and manufacturers in pharmaceutical discovery, enzymology, and carbohydrate-based diagnostic kit production, enabling precise study of glycan-processing pathways.

Application

• Enzyme Substrate for Glycosidase Assays: Used as a chromogenic substrate for high-throughput screening and kinetic studies of neuraminidases (sialidases) and other exo-glycosidases.
• Glycosyltransferase Activity Profiling: Employed in assays to measure the activity of sialyltransferases and other enzymes that modify complex carbohydrate structures.
• Diagnostic Reagent Development: Serves as a key component in the formulation of clinical diagnostic kits for detecting enzyme deficiencies or pathogen-related enzyme activity (e.g., influenza neuraminidase).
• Biochemical Research Tool: Facilitates the study of carbohydrate-protein interactions, cell surface glycan recognition, and the role of specific glycosidic linkages in biological processes.
• Inhibitor Screening: Utilized in drug discovery platforms to identify and characterize novel inhibitors of viral neuraminidases or human sialidases for therapeutic development.
• Quality Control in Biologics: Acts as a reference standard or assay component for monitoring the activity of enzyme-based biopharmaceuticals.

Basic Information

Item	Details
Product Name	Neu5Ac Alpha(2-3)Gal Beta(1-4)Glcnac-β-Pnp
CAS No.	501427-92-5
Molecular Formula	C31H45N3O21
Molecular Weight	795.70 g/mol
Synonyms	5-N-Acetylneuraminyl-α-(2→3)-galactosyl-β-(1→4)-N-acetylglucosaminyl-β-p-nitrophenyl; Neu5Acα2-3Galβ1-4GlcNAcβ-PNP; Sialyl Lewis X trisaccharide p-nitrophenyl glycoside analog; PNP-α-2,3-sialyllactosamine; Sialyl N-acetyllactosamine β-p-nitrophenyl; 3'-Sialyl-N-acetyllactosamine β-p-nitrophenyl; Chromogenic sialyl oligosaccharide substrate
EINECS	Contact for details

Quality Control

Every batch of Neu5Ac Alpha(2-3)Gal Beta(1-4)Glcnac-β-Pnp is manufactured under strict quality management systems and undergoes comprehensive analytical characterization. We ensure high purity and batch-to-batch consistency through techniques including HPLC, NMR, and mass spectrometry. Certificates of Analysis (COA) detailing purity, identity, and specific activity are provided with each shipment to support your research and regulatory needs.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This compound is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 5.0%
Specific Optical Rotation	Contact for details
Biological Activity	Contact for details

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.