Description

2'-(4-Methylumbelliferyl)-α-D-n-Acetylneuraminic Acid is a fluorogenic substrate specifically designed for the sensitive detection and quantification of neuraminidase (sialidase) enzyme activity. This compound matters as a critical research tool in virology, microbiology, and glycobiology, enabling high-throughput screening and kinetic studies. It is primarily needed by pharmaceutical R&D teams, diagnostic kit manufacturers, and academic research institutions studying influenza viruses, bacterial pathogens, and cellular glycosylation processes.

Application

• Neuraminidase (Sialidase) Activity Assays: A standard fluorogenic substrate for measuring enzyme kinetics and inhibition in high-throughput screening (HTS) platforms for antiviral drug discovery.
• Influenza Virus Research: Used to characterize viral neuraminidase subtypes and evaluate the efficacy of neuraminidase inhibitor drugs like oseltamivir and zanamivir.
• Bacterial Pathogen Detection: Employed in assays to detect sialidase activity from pathogenic bacteria such as Clostridium perfringens, Vibrio cholerae, and others.
• Diagnostic Kit Development: Serves as a key component in the formulation of commercial diagnostic kits for detecting microbial contamination or specific enzyme deficiencies.
• Glycobiology Studies: A vital reagent for investigating the role of sialic acids and their cleavage in cell signaling, cancer metastasis, and immune response.
• Quality Control in Biologics: Used to monitor and control neuraminidase activity levels during the production and purification of vaccines and other biologic therapeutics.

Basic Information

Product Name	2'-(4-Methylumbelliferyl)-α-D-n-Acetylneuraminic Acid
CAS No.	59322-44-0
Molecular Formula	C21H25NO11
Molecular Weight	467.42 g/mol
Synonyms	4-MU-NANA; 4-Methylumbelliferyl N-acetyl-α-D-neuraminic acid; 2'-(4-Methylumbelliferyl)-α-D-N-acetylneuraminic acid; MUNANA; 4-Methylumbelliferyl-α-D-N-acetylneuraminate; Fluorogenic Neuraminidase Substrate; Neuraminidase Substrate MU-NANA; (2S,4S,5R,6R)-5-Acetamido-2-[(1R,2R)-1,2-dihydroxyethyl]-6-[(7-hydroxy-2-oxo-2H-chromen-4-yl)oxy]oxane-3,4-diol
EINECS	Contact for details

Quality Control

Our products undergo rigorous quality testing to ensure compliance with industry standards for research and diagnostic use. Each batch is characterized using advanced analytical techniques including HPLC, NMR, and mass spectrometry to confirm identity, purity, and functional activity. Certificates of Analysis (COA) detailing purity, assay performance, and impurity profiles are available upon request. We adhere to a quality management system to ensure batch-to-batch consistency and reliability.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Assay (Functional)	≥ 90% of reference activity
Water Content (KF)	≤ 5.0%
Solubility	Soluble in DMSO, water/methanol mixtures

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.