Description

Uracil Dna Glycosylase is a highly specific DNA repair enzyme that catalyzes the removal of uracil residues from DNA, a critical step in the base excision repair (BER) pathway. This enzymatic activity is essential for maintaining genomic integrity by correcting mutagenic and cytotoxic lesions caused by cytosine deamination or misincorporation of dUTP. It is a fundamental reagent for molecular biology research, diagnostics, and biopharmaceutical development, particularly in next-generation sequencing (NGS) library preparation, PCR-based assays, and studies of DNA damage and repair mechanisms.

Application

• Next-Generation Sequencing (NGS): Used in library preparation protocols, such as Illumina platforms, to excise uracil bases, enabling strand-specific sequencing and reducing sequencing artifacts.
• PCR and qPCR Assay Optimization: Employed to prevent carryover contamination by degrading uracil-containing PCR products from previous amplification reactions (e.g., in dUTP-based systems).
• DNA Repair Mechanism Studies: A key tool for in vitro and in vivo research into the Base Excision Repair (BER) pathway, DNA damage response, and genomic stability.
• Molecular Diagnostics: Integral to diagnostic kits that detect genetic mutations or pathogens, where high-fidelity DNA processing is required.
• Biopharmaceutical Quality Control: Used in assays to monitor and control the quality of DNA-based therapeutics, vaccines, and recombinant proteins.
• Forensic DNA Analysis: Applied in techniques to improve the accuracy and reliability of DNA profiling from challenging samples.
• Antiviral and Cancer Research: Investigated for its role in cellular defense mechanisms and as a potential target for therapeutic intervention.

Basic Information

Product Name	Uracil Dna Glycosylase
CAS No.	59088-21-0
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	UDG; Uracil-DNA Glycosylase; Ung; UNG Enzyme; Uracil N-Glycosylase; DNA Uracil Glycosylase; EC 3.2.2.3; Thermolabile UDG; Uracil-DNA Glycosidase
EINECS	Contact for details

Quality Control

Our Uracil Dna Glycosylase is manufactured under strict quality systems. Each lot undergoes rigorous testing for enzymatic activity, purity, and stability to ensure it meets the high standards required for sensitive molecular biology applications. Certificates of Analysis (COA) detailing specifications such as specific activity, absence of contaminating nucleases (e.g., exonuclease, endonuclease), and storage buffer composition are available upon request.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store at -20°C ± 5°C. The product is hygroscopic (moisture-sensitive); ensure the container is sealed properly after each use to prevent moisture absorption, which can affect enzymatic activity. Avoid repeated freeze-thaw cycles.

Specification

Item	Specification
Appearance	Clear, colorless solution
Identification (Activity)	Conforms
Specific Activity	≥ 1 x 10⁶ units/mg
Purity (SDS-PAGE)	≥ 95%
Exonuclease Activity	Not detectable
Endonuclease Activity	Not detectable
RNase Activity	Not detectable
Storage Buffer	20 mM Tris-HCl, 1 mM DTT, 50 mM NaCl, 0.1 mM EDTA, 50% Glycerol, pH 8.0 @ 25°C

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.