Description

4-Methylumbelliferyl B-D-Glucosaminide is a specialized fluorogenic substrate used for the detection and quantification of N-acetyl-β-D-glucosaminidase (NAG) activity. This compound matters significantly in biochemical research and diagnostics, as the enzymatic cleavage releases the highly fluorescent 4-methylumbelliferone, enabling sensitive and quantitative assays. It is primarily needed by researchers and manufacturers in the life sciences, pharmaceutical development, and clinical diagnostics sectors for studying lysosomal function, renal injury markers, and enzyme kinetics.

Application

• Enzymatic Assay Substrate: Primary fluorogenic substrate for the detection of N-acetyl-β-D-glucosaminidase (NAG) activity in vitro.
• Biomedical Research: Used in studies of lysosomal storage disorders and cellular metabolism.
• Clinical Diagnostics: Key reagent in diagnostic kits for monitoring renal tubular damage, where urinary NAG is a biomarker.
• Pharmaceutical Screening: Employed in high-throughput screening (HTS) assays to identify potential enzyme inhibitors or activators.
• Microbiology: Used in research to study enzyme activity in various microorganisms.
• Biochemical Reagent: Serves as a critical component in research-grade enzyme assay kits and buffers.

Basic Information

Product Name	4-Methylumbelliferyl B-D-Glucosaminide
CAS No.	58989-36-9
Molecular Formula	C₁₆H₁₉NO₈
Molecular Weight	353.33 g/mol
Synonyms	4-Methylumbelliferyl N-acetyl-β-D-glucosaminide; 4-MU-β-GlcNAc; 4-Methylumbelliferyl 2-acetamido-2-deoxy-β-D-glucopyranoside; NAG Substrate; 4-MU-GlcNAc; β-N-Acetylglucosaminidase Substrate; 4-Methylumbelliferyl β-D-N-acetylglucosaminide
EINECS	Contact for details

Quality Control

Our 4-Methylumbelliferyl B-D-Glucosaminide is produced under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation, to ensure it meets the high standards required for sensitive fluorometric assays. Certificates of Analysis (COA) with detailed chromatographic data are available upon request to support your research and regulatory needs.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C in a dry environment. This product is hygroscopic (moisture-sensitive) and light-sensitive; prolonged exposure to moisture, heat, or light should be avoided to maintain stability and performance.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 2.0%
Fluorogenic Activity	Suitable for enzyme assays
Solubility	Soluble in DMSO, DMF; sparingly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.