Description

Adenosine-5''-o-(1-Thiotriphosphoric Acid), Rp-Isomer CAS NO 58976-49-1 is a chemically modified nucleotide analog where a non-bridging oxygen atom in the α-phosphate group of ATP is replaced by a sulfur atom, specifically in the Rp configuration. This modification renders it resistant to hydrolysis by many ATPases and kinases, making it an essential tool for mechanistic enzymology and signal transduction research. It is a critical biochemical reagent for researchers in pharmaceutical development, molecular biology, and biochemistry who require precise probes to study ATP-dependent processes, enzyme kinetics, and protein phosphorylation pathways.

Application

• Enzyme Kinetics & Mechanistic Studies: Used as a non-hydrolyzable or slow-turnover substrate analog to trap enzyme-substrate complexes and elucidate catalytic mechanisms of ATPases, kinases, and GTP-binding proteins.
• Signal Transduction Research: Serves as a vital tool for investigating phosphorylation cascades and nucleotide-dependent signaling pathways in cell biology.
• Pharmaceutical Screening: Employed in high-throughput screening (HTS) assays to identify and characterize potential inhibitors of ATP-utilizing enzymes, a common drug target class.
• Structural Biology: Used in X-ray crystallography and NMR studies to determine the three-dimensional structures of nucleotide-binding proteins in complex with a substrate analog.
• Biochemical Probe Development: Acts as a precursor for synthesizing affinity labels, photoaffinity probes, or fluorescently tagged analogs for specific protein targeting.
• Diagnostic Assay Development: Incorporated into kits and assays for measuring kinase or ATPase activity in clinical and research settings.

Basic Information

Product Name	Adenosine-5''-o-(1-Thiotriphosphoric Acid), Rp-Isomer
CAS No.	58976-49-1
Molecular Formula	C10H16N5O12P3S
Molecular Weight	523.31 g/mol
Synonyms	Adenosine 5'-(γ-thiotriphosphate), Rp-isomer; ATPγS, Rp-isomer; Adenosine-5'-triphosphorothioate, Rp-isomer; Rp-ATPγS; Rp-adenosine-5'-O-(3-thiotriphosphate); (Rp)-Adenosine-5'-(3-thiotriphosphate); Adenosine 5'-(trihydrogen diphosphate), P'→5'-ester with 3-thiophosphoric acid, Rp-isomer; 5'-Adenylyl (γ-thio)triphosphate, Rp-isomer
EINECS	Contact for details

Quality Control

Our Adenosine-5''-o-(1-Thiotriphosphoric Acid), Rp-Isomer is manufactured under stringent conditions suitable for research and diagnostic use. Each batch undergoes comprehensive analytical testing, including HPLC for purity and isomeric composition, to ensure it meets exacting standards for biochemical applications. A detailed Certificate of Analysis (COA) is provided, confirming identity, purity, and suitability for sensitive enzymatic assays.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive). For long-term storage, consider desiccation. Allow the vial to equilibrate to room temperature before opening to minimize moisture condensation.

Specification

Item	Specification
Appearance	White to off-white powder or lyophilized solid
Identification (HPLC)	Conforms to reference standard
Purity (HPLC)	≥ 95% (Rp-isomer)
Isomeric Purity (Rp-isomer)	≥ 98%
Water Content (KF)	≤ 3.0%
Biological Activity	Tested in standard kinase inhibition assay

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.