Description

Adenosine-5''-o-(1-Thiotriphosphoric Acid), Sp-Isomer CAS NO 58976-48-0 is a chemically modified nucleotide analog where a non-bridging oxygen atom in the α-phosphate group of ATP is replaced by a sulfur atom, specifically in the Sp configuration. This structural modification makes it a crucial tool in biochemistry and molecular biology, as it is resistant to hydrolysis by many ATPases and kinases, allowing it to act as a potent inhibitor or a substrate analog in enzymatic studies. It is an essential reagent for researchers in pharmaceutical development, enzymology, and signal transduction research who require precise probes to study ATP-dependent processes.

Application

• Enzymology Research: Used as a substrate analog to study the kinetics and mechanisms of ATP-dependent enzymes, including kinases, ATPases, and polymerases.
• Pharmaceutical Screening: Serves as a critical component in high-throughput screening assays to identify potential inhibitors of ATP-binding proteins involved in disease pathways.
• Structural Biology: Employed in X-ray crystallography and NMR studies to determine the three-dimensional structures of protein-ligand complexes.
• Signal Transduction Studies: Acts as a non-hydrolyzable probe to investigate G-protein coupled receptor (GPCR) signaling and other nucleotide-mediated cellular processes.
• Biochemical Probe Development: Utilized in the synthesis of affinity labels and photoaffinity probes for target identification and validation.
• Diagnostic Assay Development: Incorporated into diagnostic kits for measuring enzymatic activity in clinical and research settings.

Basic Information

Product Name	Adenosine-5''-o-(1-Thiotriphosphoric Acid), Sp-Isomer
CAS No.	58976-48-0
Molecular Formula	C10H16N5O12P3S
Molecular Weight	523.26 g/mol
Synonyms	Adenosine 5'-(γ-thio)triphosphate, Sp isomer; ATPγS, Sp isomer; Adenosine 5'-O-[γ-thio]triphosphate; γ-Thio-ATP; Adenosine-5'-triphosphorothioate; Sp-ATPyS; Sp-Adenosine 5'-[γ-thio]triphosphate; Adenosine 5'-(3-thiotriphosphate)
EINECS	Contact for details

Quality Control

Our Adenosine-5''-o-(1-Thiotriphosphoric Acid), Sp-Isomer is manufactured under strict quality systems suitable for research and diagnostic applications. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation, to ensure high lot-to-lot consistency and performance. Certificates of Analysis (COA) detailing purity, assay, and impurity profiles are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature before opening to minimize moisture absorption.

Specification

Item	Specification
Appearance	White to off-white powder or solid
Identification (HPLC)	Conforms
Purity (HPLC)	≥ 95%
Assay (Enzymatic)	≥ 90%
Water Content (KF)	≤ 3.0%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.