Description

3'(2')-o-(n-Formylmethionyl)Adenosine 5'-Phosphate is a chemically modified nucleotide derivative, specifically a formylated methionyl adenylate analog. This compound is of significant value in biochemical and pharmaceutical research, serving as a crucial tool for studying protein synthesis initiation, particularly in prokaryotic systems. It is primarily needed by research institutions, biotechnology companies, and pharmaceutical developers engaged in molecular biology, enzymology, and antibiotic mechanism studies.

Application

• Biochemical Research: Used as a substrate or inhibitor in studies of translation initiation, specifically investigating the function of initiation factor 2 (IF2) and formylmethionyl-tRNA binding.
• Enzymology Studies: Serves as a key reagent for characterizing aminoacyl-tRNA synthetases and other enzymes involved in the early stages of protein biosynthesis.
• Antibiotic Development: Employed in screening and mechanistic studies for novel antibiotics that target the bacterial protein synthesis machinery.
• Cell-Free Protein Synthesis Systems: A component in optimized in vitro translation systems for the production of proteins containing formylmethionine at the N-terminus.
• Metabolic Pathway Analysis: Useful as a tracer or standard in mass spectrometry-based studies of nucleotide and amino acid metabolism.

Basic Information

Product Name	3'(2')-o-(n-Formylmethionyl)Adenosine 5'-Phosphate
CAS No.	58682-53-4
Molecular Formula	C₁₇H₂₄N₇O₉P
Molecular Weight	525.39 g/mol
Synonyms	N-Formylmethionyladenylate; Formyl-Met-AMP; fMet-AMP; N-Formylmethionyl adenylate; 3'(2')-O-(N-Formyl-L-methionyl)adenosine 5'-phosphate; Initiation analog fMet-AMP; Adenosine 5'-(3-(N-formyl-L-methionyl)phosphate)
EINECS	Contact for details

Quality Control

Our 3'(2')-o-(n-Formylmethionyl)Adenosine 5'-Phosphate is produced under stringent conditions to ensure high purity and batch-to-batch consistency, suitable for sensitive research applications. Each lot undergoes comprehensive analytical testing including HPLC for purity, NMR for structural confirmation, and mass spectrometry for identity verification. Certificates of Analysis (COA) detailing all specifications are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a desiccated environment. This product is hygroscopic (moisture-sensitive) and should be allowed to equilibrate to room temperature in a dry atmosphere before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms
Identification (NMR)	Conforms
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 3.0%
Solubility	Soluble in water, DMSO

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.