Description

Glutaryl-Phe-Amc is a synthetic peptide derivative, specifically a glutaryl-modified phenylalanine amide, widely utilized as a key biochemical tool and research intermediate. Its primary value lies in its role as a selective and sensitive fluorogenic substrate for various proteolytic enzymes, particularly cathepsins and other cysteine proteases. This compound is essential for researchers and manufacturers in the fields of pharmaceutical development, biochemical assay design, and proteomics research, enabling critical studies on enzyme kinetics, inhibitor screening, and disease mechanism elucidation.

Application

• Enzyme Activity Assays: Fluorogenic substrate for the continuous, real-time monitoring of protease activity, particularly for cathepsin B, L, and H.
• Drug Discovery & Screening: High-throughput screening (HTS) of potential protease inhibitors for therapeutic development in cancer, osteoporosis, and inflammatory diseases.
• Biochemical Research: Tool for studying protease function, specificity, and regulation in cell biology and pathophysiology studies.
• Diagnostic Reagent Formulation: Key component in the development of diagnostic kits for detecting specific protease activities associated with certain disease states.
• Proteomics: Used in activity-based protein profiling (ABPP) to identify and characterize active proteases in complex biological samples.
• Academic & Institutional Research: Fundamental reagent for university and research institute laboratories focused on enzymology and molecular biology.

Basic Information

Item	Details
Product Name	Glutaryl-Phe-Amc
CAS No.	58632-47-6
Molecular Formula	C₂₁H₂₉N₃O₆
Molecular Weight	419.47 g/mol
Synonyms	Glutaryl-Phenylalanine-7-Amido-4-Methylcoumarin; Glutaryl-Phe-AMC; Glt-Phe-AMC; Glutaryl-L-Phenylalanine 7-Amido-4-Methylcoumarin; Cathepsin B Substrate (Glutaryl-Phe-AMC); Z-Gly-Pro-AMC (related class); Protease Fluorogenic Substrate V; L-Glutaryl-L-Phenylalanyl-7-Amido-4-Methylcoumarin
EINECS	Contact for details

Quality Control

Our Glutaryl-Phe-Amc is produced under strict quality management systems to ensure batch-to-batch consistency and high purity for research applications. Each lot undergoes comprehensive analytical testing, including HPLC for purity verification, mass spectrometry (MS) for identity confirmation, and NMR for structural validation. A detailed Certificate of Analysis (COA) documenting identity, purity (typically ≥95% by HPLC), and specific performance data is provided with every shipment to support your quality assurance protocols.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white powder or solid
Identification (HPLC/MS)	Conforms to reference standard
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 5.0%
Solubility	Soluble in DMSO, DMF; sparingly soluble in aqueous buffers
Functional Activity	Contact for details

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.